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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sodium and water intake are not affected by GABA(C) receptor activation in the lateral parabrachial nucleus of sodium-depleted rats

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Domingos-Souz, Gean [1] ; Meschiari, Cesar Arruda [1] ; Buzelle, Samyra Lopes [1] ; Callera, Joao Carlos [2] ; Antunes-Rodrigues, Jose [1]
Total Authors: 5
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] UNESP Univ Estadual Paulista, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Chemical Neuroanatomy; v. 74, p. 47-54, JUL 2016.
Web of Science Citations: 1

The activation of GABAergic receptors, GABA(A) and GABA(B), in the lateral parabrachial nucleus (LPBN) increases water and sodium intake in satiated and fluid-depleted rats. The present study investigated the presence of the GABA(C) receptor in the LPBN, its involvement in water and sodium intake, and its effects on cardiovascular parameters during the acute fluid depletion induced by furosemide combined with captopril (Furo/Cap). One group of male Wistar rats (290-300 g) with bilateral stainless steel LPBN cannulas was used to test the effects of a GABA(C) receptor agonist and antagonist on the fluid intake and cardiovascular parameters. We investigated the effects of bilateral LPBN injections of trans-4-aminocrotonic acid (TACA) on the intake of water and 0.3 M NaCl induced by acute fluid depletion (subcutaneous injection of Furo/Cap). c-Fos expression increased (P<0.05), suggesting LPBN neuronal activation. The injection of different doses of TACA (0.5, 2.0 and 160 nmol) in the LPBN did not change the sodium or water intake in Furo/Cap-treated rats (P>0.05). Treatment with the GABA(C) receptor antagonist (Z)-3-{[}(aminoiminomethyl)thio]prop-2-enoic acid sulfate (ZAPA, 10 nmol) or with ZAPA (10 nmol) plus TACA (160 nmol) did not change the sodium or water intake compared with that for vehicle (saline) (P >0.05). Bilateral injections of the GABA(C) agonist in the LPBN of Furo/Cap-treated rats did not affect the mean arterial pressure (MAP) or heart rate (HR). The GABA(C) receptor expression in the LPBN was confirmed by the presence of a 50 kDa band. Although LPBN neurons might express GABA(C) receptors, their activation produced no change in water and sodium intake or in the cardiovascular parameters in the acute fluid depletion rats. Therefore, the GABA(C) receptors in the LPBN might not interfere with fluid and blood pressure regulation. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/03006-4 - Cardiovascular and hypertonic NaCl and water intake effects induced by GABAC agonist injected into the lateral parabrachial nucleus (LPBN) in rats
Grantee:Gean Domingos da Silva Souza
Support type: Scholarships in Brazil - Master