Long-term in vivo polychlorinated biphenyl 126 exposure induces oxidative stress and alters proteomic profile on islets of Langerhans

Author(s)

Loiola, Rodrigo Azevedo ^[1] ; dos Anjos, Fabyana Maria ^[1] ; Shimada, Ana Lucia ^[1] ; Cruz, Wesley Soares ^[1] ; Drewes, Carine Cristiane ^[1] ; Rodrigues, Stephen Fernandes ^[1] ; Cardozo, Karina Helena Morais ^[2] ; Carvalho, Valdemir Melechco ^[2] ; Pinto, Ernani ^[1] ; Farsky, Sandra Helena ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Analyses, Sao Paulo - Brazil

^[2] Fleury Grp, Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 6, JUN 13 2016.
• Web of Science Citations: 6

Abstract

It has been recently proposed that exposure to polychlorinated biphenyls (PCBs) is a risk factor to type 2 diabetes mellitus (DM2). We investigated this hypothesis using long-term in vivo PCB126 exposure to rats addressing metabolic, cellular and proteomic parameters. Male Wistar rats were exposed to PCB126 (0.1, 1 or 10 mu g/kg of body weight/ day; for 15 days) or vehicle by intranasal instillation. Systemic alterations were quantified by body weight, insulin and glucose tolerance, and blood biochemical profile. Pancreatic toxicity was measured by inflammatory parameters, cell viability and cycle, free radical generation, and proteomic profile on islets of Langerhans. In vivo PCB126 exposure enhanced the body weight gain, impaired insulin sensitivity, reduced adipose tissue deposit, and elevated serum triglycerides, cholesterol, and insulin levels. Inflammatory parameters in the pancreas and cell morphology, viability and cycle were not altered in islets of Langerhans. Nevertheless, in vivo PCB126 exposure increased free radical generation and modified the expression of proteins related to oxidative stress on islets of Langerhans, which are indicative of early beta-cell failure. Data herein obtained show that long-term in vivo PCB126 exposure through intranasal route induced alterations on islets of Langerhans related to early end points of DM2. (AU)

• FAPESP's process: 12/02994-0 - Experimental study of PCB126 exposure on induction type II Diabetes mellitus
• Grantee: Sandra Helena Poliselli Farsky
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/09677-8 - Experimental study of exposure to PCB126 on induction of type II Diabetes Mellitus
• Grantee: Ana Lucia Borges Shimada
• Support Opportunities: Scholarships in Brazil - Doctorate