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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

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Author(s):
Biscola, Natalia Perussi [1, 2] ; Cartarozzi, Luciana Politti [3] ; Ferreira Junior, Rui Seabra [1, 2] ; Barraviera, Benedito [1, 2] ; Rodrigues de Oliveira, Alexandre Leite [3]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Trop Dis, BR-18618000 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, BR-2CENTER Botucatu, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: NEURAL PLASTICITY; 2016.
Web of Science Citations: 8
Abstract

Brachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA) without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS) at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons. (AU)

FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/53846-9 - Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant
Grantee:Benedito Barraviera
Support type: Multi-user Equipment Program
FAPESP's process: 11/23377-7 - Neuroprotective and regenerative effects of fibrin sealant derived from snake venom combined with mononuclear stem cells after neurorrhaphy of the sciatic nerve of neonatal rats
Grantee:Natália Perussi Biscola
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/06892-3 - Use of mesenchymal stem cells in the CNS/PNS interface: repair of proximal lesions
Grantee:Alexandre Leite Rodrigues de Oliveira
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/08101-8 - In vivo imaging of nerve regeneration following ventral root replantation with fluo-stained fibrin sealant associated with mesenchymal stem cells
Grantee:Rui Seabra Ferreira Junior
Support type: Regular Research Grants