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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xylanase and β-xylosidase from Penicillium janczewskii: Purification, characterization and hydrolysis of substrates

Full text
Author(s):
César Rafael Fanchini Terrasan ; José Manuel Guisan ; Eleonora Cano Carmona
Total Authors: 3
Document type: Journal article
Source: ELECTRONIC JOURNAL OF BIOTECHNOLOGY; v. 19, n. 5, p. 54-62, Set. 2016.
Abstract

Background: Xylanases and β-D-xylosidases are the most important enzymes responsible for the degradation of xylan, the second main constituent of plant cell walls. Results: In this study, the main extracellular xylanase (XYL I) and p-xylosidase (BXYL I) from the fungus Penicillium janczewskii were purified, characterized and applied for the hydrolysis of different substrates. Their molecular weights under denaturing and non-denaturing conditions were, respectively, 30.4 and 23.6 kDa for XYL I, and 100 and 200 kDa for BXYL I, indicating that the latter is homodimeric. XYL I is highly glycosylated (78%) with optimal activity in pH 6.0 at 65°C, while BXYL I presented lower sugar content (10.5%) and optimal activity in pH 5.0 at 75°C. The half-lives of XYL I at 55, 60 and 65°C were 125,16 and 6 min, respectively. At 60°C, BXYL I retained almost 100% of the activity after 6 h. NH4+,Na+, DTT and β-mercaptoethanol stimulated XYL I, while activation of BXYL I was not observed. Interestingly, XYL I was only partially inhibited by Hg2+, while BXYL I was completely inhibited. Xylobiose, xylotriose and larger xylooligosaccharides were the main products from xylan hydrolysis by XYL I. BXYL I hydrolyzed xylobiose and larger xylooligosaccharides with no activity against xylans. Conclusion: The enzymes act synergistically in the degradation of xylans, and present industrial characteristics especially in relation to optimal activity at high temperatures, prolonged stability of BXYL I at 60°C, and stability of XYL I in wide pH range. (AU)

FAPESP's process: 07/08640-8 - Xylanases, b-xylosidases from Penicillium janczewskii: purification, characterization and application in cellulosic pulp bleaching and for animal feed
Grantee:César Rafael Fanchini Terrasan
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/16582-0 - Purification, properties and application of xylanolytic enzymes produced by Penicillium janczewskii
Grantee:Eleonora Cano Carmona
Support type: Regular Research Grants