Study of spatial and temporal distribution of antimicrobial in water and sediments from caging fish farms by on-line SPE-LC-MS/MS

Author(s)

Monteiro, Sergio H. ; Francisco, Jeane G. ; Andrade, Graziela C. R. M. ; Botelho, Rafael G. ; Figueiredo, Leila A. ; Tornisielo, Valdemar L.

• Document type: Journal article
• Source: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN; v. 51, n. 9, p. 634-643, 2016.
• Web of Science Citations: 6

Abstract

An on-line solid phase extraction liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method for the determination of 12 antimicrobials in sediment and surface water was developed and validated. Furthermore, the spatial and temporal antimicrobials distributions in the sediment and in the water of four fish farms located in the hydroelectric dam of Ilha Solteira Reservoir in Brazil were investigated over four seasons in three sampling sites: at the fish cages, 100 and 1,000m downstream far from the cages. The method was performed using an Agilent Zorbax 80 SB-C8 column (9.4 x 15mm, 5 mu m) as the loading column, and the Agilent Zorbax Eclipse Plus C18 column (3.0 x 100mm, 3.5 mu m) as a separation column within a run time of 13min. The limits of quantification were less than 9ngL(-1) for the antibiotics in water and 16 mu gkg(-1) in sediment; the recovery ranged from 80 to 119%, with a variation coefficient less than 11%, and the repeatability was lower than 15%. Oxytetracycline was found in the water in all sample seasons. However, florfenicol was identified in April and October 2013 and January 2014, and tetracycline was present in July 2013. Regarding the sediment, oxytetracycline and tetracycline were found in all sampling periods, but chlortetracycline was only identified in January 2014. The spatial distribution of antimicrobials showed that the main pollution source came from the fish farms. This study demonstrated that the proposed method is reliable for the monitoring of antimicrobials in water and sediments and it showed contamination in both matrices from Ilha Solteira Reservoir. (AU)

• FAPESP's process: 11/18179-1 - Occurrence of antibiotics and microbial resistance study in aquaculture systems of the Paraná River, Ilha Solteira reservoir, in the Santa Fé do Sul region, São Paulo
• Grantee: Valdemar Luiz Tornisielo
• Support Opportunities: Regular Research Grants