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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cross-Linking Mast Cell Specific Gangliosides Stimulates the Release of Newly Formed Lipid Mediators and Newly Synthesized Cytokines

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Author(s):
Freitas Filho, Edismauro Garcia ; Marcelino da Silva, Elaine Zayas ; Zanotto, Camila Ziliotto ; Oliver, Constance ; Jamur, Andmaria Celia
Total Authors: 5
Document type: Journal article
Source: Mediators of Inflammation; 2016.
Web of Science Citations: 3
Abstract

Mast cells are immunoregulatory cells that participate in inflammatory processes. Cross-linking mast cell specific GD1b derived gangliosides by mAbAA4 results in partial activation of mast cells without the release of preformed mediators. The present study examines the release of newly formed and newly synthesized mediators following ganglioside cross-linking. Cross-linking the gangliosides with mAbAA4 released the newly formed lipid mediators, prostaglandins D-2 and E-2, without release of leukotrienes B-4 and C 4. The effect of cross-linking these gangliosides on the activation of enzymes in the arachidonate cascade was then investigated. Ganglioside cross-linking resulted in phosphorylation of cytosolic phospholipase A(2) and increased expression of cyclooxygenase-2. Translocation of 5-lipoxygenase from the cytosol to the nucleus was not induced by ganglioside cross-linking. Cross-linking of GD1b derived gangliosides also resulted in the release of the newly synthesized mediators, interleukin-4, interleukin-6, and TNF-alpha. The effect of cross-linking the gangliosides on the MAP kinase pathway was then investigated. Cross-linking the gangliosides induced the phosphorylation of ERK1/2, JNK1/2, and p38 as well as activating both NF kappa B and NFAT in a Syk-dependent manner. Therefore, cross-linking the mast cell specific GD1b derived gangliosides results in the activation of signaling pathways that culminate with the release of newly formed and newly synthesized mediators. (AU)

FAPESP's process: 14/17671-8 - Mast cell specific gangliosides modulate mediator release
Grantee:Maria Célia Jamur
Support type: Regular Research Grants
FAPESP's process: 12/06373-0 - The involvement of the adaptor protein complex AP-3 in mast cell regulated secretion
Grantee:Elaine Zayas Marcelino da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/12861-0 - The role of mast cell specific gangliosides in modulating mediator release
Grantee:Edismauro Garcia Freitas Filho
Support type: Scholarships in Brazil - Master