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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

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Mimura, Kallyne K. O. ; Moraes, Andreia R. ; Miranda, Aline C. ; Greco, Rebecca ; Ansari, Tahera ; Sibbons, Paul ; Greco, Karin V. ; Oliani, Sonia M.
Total Authors: 8
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 6, OCT 11 2016.
Web of Science Citations: 10

Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair. (AU)

FAPESP's process: 12/13041-4 - Evaluation of skin heterologous graft using acellular matrices obtained from porcine skin and annexin A1 action as anti-inflammatory / immunosuppressive agent.
Grantee:Kallyne Kioko Oliveira Mimura
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/21603-2 - Tissue bioengineering: evaluate skin xenograft using porcine acellular matrices and effect of annexin A1 protein as a therapeutic approach in the regenerative and wound healing processes
Grantee:Sonia Maria Oliani
Support Opportunities: Regular Research Grants
FAPESP's process: 14/18557-4 - Study of inflammatory and angiogenic processes in heterologous implants of rats using porcine acellular matrices
Grantee:Andréia Rodrigues de Moraes
Support Opportunities: Scholarships in Brazil - Scientific Initiation