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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Therapy with resveratrol attenuates obesity-associated allergic airway inflammation in mice

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Author(s):
Andre, Diana Majolli ; Calixto, Marina Ciarallo ; Sollon, Carolina ; Alexandre, Eduardo Costa ; Leiria, Luiz O. ; Tobar, Natalia ; Anhe, Gabriel Forato ; Antunes, Edson
Total Authors: 8
Document type: Journal article
Source: International Immunopharmacology; v. 38, p. 298-305, SEP 2016.
Web of Science Citations: 17
Abstract

Obesity and insulin resistance have been associated with deterioration in asthma outcomes. High oxidative stress and deficient activation of AMP-activated protein kinase (AMPIC) have emerged as important regulators linking insulin resistance and inflammation. This study aimed to evaluate the effects of resveratrol on obesity-associated allergic pulmonary inflammation. Male C57/B16 mice fed with high-fat diet to induce obesity (obese group) or standard-chow diet (lean group) were treated or not with resveratrol (100 mg/kg/day, two weeks). Mice were sensitized and challenged with ovalbumin (OVA). At 48 h thereafter, bronchoalveolar lavage fluid was performed, and lungs collected for morphological studies and Western blot analysis. Treatment of obese mice with resveratrol significantly reduced hyperglycemia and insulin resistance, as well as the body measures (body mass, fat mass, % fat, and body area). OVA-challenge promoted a higher increase in pulmonary eosinophil infiltration in obese compared with lean mice, which was nearly abrogated by resveratrol treatment. Resveratrol markedly increased the phosphorylated AMPIC expression in lung tissues of obese compared with lean mice. Resveratrol reduced the p47phox expression and reactive-oxygen species (ROS) production, and elevated the superoxide dismutase (SOD) levels in lung tissues of obese mice. The increased pulmonary levels of TNF-alpha and inducible nitric oxide synthase (iNOS) in obese mice were also normalized after resveratrol treatment. In lean mice, resveratrol failed to affect the levels of fasting glucose, p47phox, ROS levels, TNF-alpha, iNOS and phosphorylated AMPIC. Resveratrol exhibits protective effects in obesity-associated lung inflammation that is accompanied by local AMPIC activation and antioxidant property. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/02130-1 - Role of insulin resistance in the pulmonary eosinophil recruitment in obese mice: anti-inflammatory and antioxidant actions of resveratrol
Grantee:Edson Antunes
Support type: Regular Research Grants