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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of in vitro exposure to butylparaben and di-(2 ethylhexyl) phthalate, alone or in combination, on ovarian function

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Author(s):
Guerra, Marina T. ; Furlong, Hayley C. ; Kempinas, Wilma G. ; Foster, Warren G.
Total Authors: 4
Document type: Journal article
Source: JOURNAL OF APPLIED TOXICOLOGY; v. 36, n. 9, p. 1235-1245, SEP 2016.
Web of Science Citations: 4
Abstract

Parabens and phthalates are commercial chemicals widely used in the manufacture of industrial and consumer products frequently found as contaminants in biological fluids. We evaluated the effects of di-(2-ethylhexyl) phthalate (DEHP) (ranging from 10(-9) to 10(-7) m {[}1-100 nm; 0.39-39 ng ml(-1)]) and butylparaben (BP) (ranging from 10(-8) to 10(-5) m {[}10 nm-10 m; 1.9 ng ml(-1) to 1.9 g ml(-1)]), alone and in combination, on isolated mouse preantral follicle and human granulosa cell (hGC) cultures to study direct effects on follicle growth and ovarian steroidogenesis. Our results revealed that, in follicle culture, DEHP and BP attenuate estradiol output but only when present together. DEHP decreases progesterone concentrations in the spent media of hGC cultures, an effect that was attenuated when BP was added together with DEHP. Although changes in steroidogenesis were observed, no effects on follicular development or survival were noted in the culture systems. We suggest that BP and DEHP act with additive effect to decrease estradiol production whereas at later stages of follicle development BP blocks the effect of DEHP in hGCs resulting in decreased progesterone output. Taken together our results suggest that DEHP and BP adversely affect steroidogenesis from the preantral stage onward and the effects of these chemicals are both stage-dependent and modified by co-exposure. Copyright (c) 2016 John Wiley \& Sons, Ltd. We evaluated the effects of Di-(2-ethylhexyl) phthalate (DEHP) and Butylparaben (BP), alone and in combination, on isolated mouse preantral follicle and human granulosa cell (hGC) cultures to study direct effects on follicle growth and ovarian steroidogenesis. Our results suggest that DEHP and BP adversely affect steroidogenesis from the preanatral stage onward and the effects of these chemicals are both stage-dependent and modified by co-exposure. (AU)

FAPESP's process: 14/09409-1 - Evaluation of the possible effects of in vitro exposure to butylparaben and di-(2-ethylhexyl) phthalate, alone or in association on steroidogenesis
Grantee:Marina Trevizan Guerra
Support type: Scholarships abroad - Research Internship - Post-doctor