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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Low-level laser therapy induces an upregulation of collagen gene expression during the initial process of bone healing: a microarray analysis

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Tim, Carla Roberta ; Bossini, Paulo Sergio ; Kido, Hueliton Wilian ; Malavazi, Iran ; Kress, Marcia Regina von Zeska ; Carazzolle, Marcelo Falsarella ; Renno, Ana Claudia ; Parizotto, Nivaldo Antonio
Total Authors: 8
Document type: Journal article
Source: Journal of Biomedical Optics; v. 21, n. 8 AUG 2016.
Web of Science Citations: 4

This study investigates the histological modifications produced by low level laser therapy (LLLT) on the first day of bone repair, as well as evaluates the LLLT effects on collagen expression on the site of a fracture. Twenty Wistar rats were distributed into a control group (CG) and a laser group (LG). Laser irradiation of Ga-AlAs laser 830 nm, 30 mW, 94 s, 2.8 J was performed in five sessions. Animals were euthanized on day 5 post-surgery. Histopathological analysis showed that LLLT was able to increase deposition of granulation tissue and newly formed bone at the site of the injury. In addition, picrosirius analysis showed that collagen fiber organization in the LG was enhanced compared to CG. Microarray analysis demonstrated that LLLT produced an upregulation type I collagen (COL-I). Immunohistochemical analysis revealed that the subjects that were treated presented a higher immunoexpression of COL-I. Our findings indicated that LLLT improves bone healing by producing a significant increase in the expression of collagen genes. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

FAPESP's process: 10/15335-0 - Analysis of gene expression during bone repair defects in tibia irradiated with low intensity laser therapy.
Grantee:Carla Roberta Tim
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
Grantee:Munir Salomao Skaf
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC