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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improved catalytic properties of Candida antarctica lipase B multi-attached on tailor-made hydrophobic silica containing octyl and multifunctional amino- glutaraldehyde spacer arms

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Author(s):
Vescovi, Vinicius ; Kopp, Willian ; Guisan, Jose M. ; Giordano, Raquel L. C. ; Mendes, Adriano A. ; Tardioli, Paulo W.
Total Authors: 6
Document type: Journal article
Source: Process Biochemistry; v. 51, n. 12, p. 2055-2066, DEC 2016.
Web of Science Citations: 20
Abstract

This work evaluated the immobilization of Candida antarctica lipase B (CALB) on a new heterofunctional support, prepared by silanization of silica particles with triethoxy(octyl)silane and trietoxy(3-aminopropyl)silane, followed by activation with glutaraldehyde. The new support (octyl-silica-amino-glutaraldehyde, OSGlu) exhibits features of an anionic exchanger and hydrophobic adsorbent capable of adsorbing and covalently linking the lipase under mild conditions in a single step. As comparison, CALB was also immobilized on silica coated with octyl, amino-glutaraldehyde, octyl and glyoxyl, and octyl and epoxy groups. Among these supports, CALB immobilized on OSGlu (namely CALB-OSGlu), showed the highest recovered activity (71 +/- 2%) and thermal stability in tert-butyl alcohol (full activity was recovered after 100 h at 65 degrees C). CALK immobilized on silica coated with octyl or amino-glutaraldehyde yielded biocatalysts with lower recovered activities, compared to CALB-OSGlu, with values of 43% and 32%, respectively. CALB-OSGlu was chosen to be evaluated in the synthesis of fructose oleate in tert-butyl alcohol medium and at high temperature, achieving conversions above 70% in nine 6 h-cycles at 55 degrees C. CALB-OSGlu showed better operational stability than benchmark immobilized CALB.(Novozym 435) in the same reaction conditions, with the conversion of the later decreasing from 84% (first cycle) to 53% (ninth cycle). (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/23194-0 - Enzymatic synthesis of fatty esters using heating assisted by microwave
Grantee:Paulo Waldir Tardioli
Support Opportunities: Regular Research Grants
FAPESP's process: 15/12980-5 - ECCE 10 + ECAB 3 + EPIC 5
Grantee:Paulo Waldir Tardioli
Support Opportunities: Research Grants - Meeting - Abroad