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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genome-Wide Association Study of Cell-Mediated Response in Dogs Naturally Infected by Leishmania infantum

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Batista, Luis F. S. ; Utsunomiya, Yuri T. ; Silva, Thais B. F. ; Dias, Raissa A. ; Tomokane, Thaise Y. ; Pacheco, Acacio D. ; da Matta, Vania L. R. ; Silveira, Fernando T. ; Marcondes, Mary ; Nunes, Caris M. ; Laurenti, Marcia D.
Total Authors: 11
Document type: Journal article
Source: Infection and Immunity; v. 84, n. 12, p. 3629-3637, DEC 2016.
Web of Science Citations: 2
Abstract

A genome-wide association study (GWAS) could unravel the complexity of the cell-mediated immunity (CMI) to canine leishmaniasis (CanL). Therefore, we scanned 110,165 single-nucleotide polymorphisms (SNPs), aiming to identify chromosomal regions associated with the leishmanin skin test (LST), lymphocyte proliferation assay (LPA), and cytokine responses to further understand the role played by CMI in the outcome of natural Leishmania infantum infection in 189 dogs. Based on LST and LPA, four CMI profiles were identified (LST-/LPA(-), LST+ /LPA(-), LST- /LPA(+), and LST+ /LPA(+)), which were not associated with subclinically infected or diseased dogs. LST+/LPA(+) dogs showed increased interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) levels and mild parasitism in the lymph nodes, whereas LST-/ LPA(+) dogs, in spite of increased IFN-beta, also showed increased interleukin-10 (IL-10) and transforming growth factor beta (TGF-beta) levels and the highest parasite load in lymph nodes. Low T cell proliferation under low parasite load suggested that L. infantum was not able to induce effective CMI in the early stage of infection. Altogether, genetic markers explained 87%, 16%, 15%, 11%, 0%, and 0% of phenotypic variance in TNF-alpha, TGF-beta, LST, IL-10, IFN-gamma, and LPA, respectively. GWAS showed that regions associated with TNF-alpha include the following genes: IL12RB1, JAK3, CCRL2, CCR2, CCR3, and CXCR6, involved in cytokine and chemokine signaling; regions associated with LST, including COMMD5 and SHARPIN, involved in regulation of NF-kappa B signaling; and regions associated with IL-10, including LTBP1 and RASGRP3, involved in T regulatory lymphocytes differentiation. These findings pinpoint chromosomic regions related to the cell-mediated response that potentially affect the clinical complexity and the parasite replication in canine L. infantum infection. (AU)

FAPESP's process: 12/05847-9 - Clinical, immunological evaluation and their correlation with genetic markers in canine visceral leishmaniasis
Grantee:Luís Fábio da Silva Batista
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/01095-8 - Use of next generation sequencing for the fine mapping of loci explaining variance in scrotal circumference in Nellore cattle
Grantee:Yuri Tani Utsunomiya
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/50285-9 - Clinical, immunological features and its correlation with genetic markers in canine visceral leishmaniasis
Grantee:Marcia Dalastra Laurenti
Support type: Regular Research Grants