The SURMetaGIT study: Design and rationale for a prospective pan-omics examination of the gastrointestinal response to Roux-en-Y gastric bypass surgery

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Sala, Priscila ; Belarmino, Giliane ; Machado, Natasha Mendonca ; Cardinelli, Camila Siqueira ; Al Assal, Karina ; Silva, Mariane Marques ; Fonseca, Danielle Cristina ; Ishida, Robson Kiyoshi ; Santo, Marco Aurelio ; Hourneaux de Moura, Eduardo Guimaraes ; Sakai, Paulo ; Mota Siqueira Guarda, Ismael Francisco ; Cotrim Guerreiro da Silva, Ismael Dale ; Rodrigues, Agatha Sacramento ; de Braganca Pereira, Carlos Alberto ; Heymsfield, Steven ; Dore, Joel ; Matos de Miranda Torrinhas, Raquel Susana ; Giannella-Neto, Daniel ; Waitzberg, Dan Linetzky

• Document type: Journal article
• Source: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH; v. 44, n. 6, p. 1359-1375, DEC 2016.
• Web of Science Citations: 4

Abstract

Objective: To describe the protocol of the SURgically induced Metabolic effects on the Human GastroIntestinal Tract (SURMetaGIT) study, a clinical pan-omics study exploring the gastrointestinal tract as a central organ driving remission of type 2 diabetes mellitus (T2DM) after Roux-en-Y gastric bypass (RYGB). The main points considered in the study's design and challenges faced in its application are detailed. Methods: This observational, longitudinal, prospective study involved collection of gastrointestinal biopsy specimens, faeces, urine, and blood from 25 obese women with T2DM who were candidates for RYGB (20 patients for omics assessment and 5 for omics validation). These collections were performed preoperatively and 3 and 24 months postoperatively. Gastrointestinal transcriptomics; faecal metagenomics and metabolomics; plasma proteomics, lipidomics, and metabolomics; and biochemical, nutritional, and metabolic data were assessed to identify their short- and long-term correlations with T2DM remission. Results: Data were collected from 20 patients before and 3 months after RYGB. These patients have nearly completed the 2-year follow-up assessments. The five additional patients are currently being selected for omics data validation. Conclusion: The multi-integrated pan-omics approach of the SURMetaGIT study enables integrated analysis of data that will contribute to the understanding of molecular mechanisms involved in T2DM remission after RYGB. (AU)

• FAPESP's process: 15/22820-5 - Short and long term evaluation of caloric intake and macro and micronutrients on obese pacients carriers of Melittus type 2 after bypass surgery Y - Roux
• Grantee: Regiane Macedo Silva Dippolito
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/09612-3 - Changes in gene expression in gastric and intestinal tissue of type 2 Diabetes patients submitted to Roux-en-Y gastric bypass
• Grantee: Dan Linetzky Waitzberg
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/05220-1 - Metabolomics of fecal water in obese patients with Type 2 Diabetes before and after gastroplasty reduction in Y-Roux (GRYR)
• Grantee: Camila de Siqueira Cardinelli
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 13/03246-0 - Genomic, proteomic and metabolomic study of gastrointestinal tissue for patients with type 2 Diabetes mellitus after Roux-en-Y gastric bypass
• Grantee: Priscila Sala Kobal
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 14/07742-5 - Genomic, proteomic and metabolomic study of gastrointestinal tissue for patients with type 2 diabetes mellitus after Roux-en-Y gastric bypass
• Grantee: Priscila Sala Kobal
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 12/23762-0 - Assessment of food intake and comparison of 24h food recall and food record of 7 days applied in obese patients in the preoperative and postoperative of Roux-en-Y Gastric Bypass
• Grantee: Mariane Marques da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/01259-6 - Metabolomic profile of patients with type 2 diabetes and obesity after Roux en Y gastric bypass
• Grantee: Natasha Mendonça Machado
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 13/23355-9 - Metabolomics profile of patients with type 2 diabetes and obesity after Roux en Y gastric bypass
• Grantee: Natasha Mendonça Machado
• Support Opportunities: Scholarships in Brazil - Doctorate