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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oral cavities of healthy infants harbour high proportions of Streptococcus salivarius strains with phenotypic and genotypic resistance to multiple classes of antibiotics

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Palma, Thais H. ; Harth-Chu, Erika N. ; Scott, Jodie ; Stipp, Rafael N. ; Boisvert, Heike ; Salomao, Mariana F. ; Theobaldo, Jessica D. ; Possobon, Rosana F. ; Nascimento, Leandro C. ; McCafferty, Jonathan W. ; Faller, Lina ; Duncan, Margaret J. ; Mattos-Graner, Renata O.
Total Authors: 13
Document type: Journal article
Source: Journal of Medical Microbiology; v. 65, n. 12, p. 1456-1464, DEC 2016.
Web of Science Citations: 4
Abstract

Emerging antibiotic resistance in the oropharyngeal microbiota, of which Streptococcus salivarius is a prominent species, represents a challenge for treating paediatric populations. In this study, we investigated the role of Streptococcus salivarius as a reservoir for antibiotic resistance genes (ARG) in the oral microbiota by analysing 95 Streptococcus salivarius isolates from 22 healthy infants (2-16 months of age). MICs of penicillin G, amoxicillin, erythromycin, tetracycline, doxycycline and streptomycin were determined. ARG profiles were assessed in a subset of 21 strains by next-generation sequencing of genomes, followed by searches of assembled reads against the Comprehensive Antibiotic Resistance Database. Strains resistant to erythromycin, penicillins and tetracyclines were isolated from 83.3, 33.3 and 16.6 %, respectively, of infants aged 2 to 8 months with no prior antibiotic treatment. These percentages were 100.0, 66.6 and 50.0 %, by 13 to 16 months of age. ARG or polymorphisms associated with antibiotic resistance were the most prevalent and involved genes for macrolide efflux (mel, mefA/E and macB), ribosomal protection {[}erm(B), tet(M) and tet(O)] and beta-lactamase-like proteins. Phylogenetically related strains showing multidrug-resistant phenotypes harboured multidrug efflux ARG. Polymorphic genes associated with antibiotic resistance to drugs affecting DNA replication, folate synthesis, RNA/protein synthesis and regulators of antibiotic stress responses were detected. These data imply that Streptococcus salivarius strains established during maturation of the oral microbiota harbour a diverse array of functional ARG, even in the absence of antibiotic selective pressures, highlighting a potential role for this species in shaping antibiotic susceptibility profiles of oropharyngeal communities. (AU)

FAPESP's process: 15/12940-3 - Identification of surface proteins of Streptococcus mutans involved in evasion of opsonization by the complement system
Grantee:Renata de Oliveira Mattos Graner
Support type: Regular Research Grants
FAPESP's process: 11/09761-9 - Analysis of patterns of antibiotic sensitivity in pioneer strains of Streptococcus of the human oral cavity.
Grantee:Thaís Harder de Palma
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 09/50547-0 - Characterization of GbpB/PcsB homologues in commensal species of oral streptococci
Grantee:Erika Nikitza Shiauha Harth Chu
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/06786-8 - Analysis of oral colonization by non-hemolytic species of the genus Streptococcus in children of 2 to 16 months of age
Grantee:Mariana da Fonseca
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/51832-3 - Analysis of a new Two Component System possibly involved in the adaptation of Streptococcus mutans and Streptococcus sanguinis to human saliva.
Grantee:Renata de Oliveira Mattos Graner
Support type: Regular Research Grants
FAPESP's process: 13/06820-0 - Analysis of the patterns of antibiotic sensitivity in pioneer strains of streptococci from the oral cavity
Grantee:Thaís Harder de Palma
Support type: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 10/06785-1 - Phylogenetic analysis of Streptococcal strains of the groups Mitis and Sanguinis isolated from children during initial stages of oral colonization
Grantee:Jessica Dias Theobaldo
Support type: Scholarships in Brazil - Scientific Initiation