MicroRNA expression signatures in lungs of mice infected with Mycobacterium tuberculosis

Author(s):Show less -

Malardo, Thiago ; Gardinassi, Luiz Gustavo ; Moreira, Bernardo Pereira ; Padilha, Everton ; Cetrulo Lorenzi, Julio Cesar ; Soares, Luana Silva ; Gembre, Ana Flavia ; Fontoura, Isabela Cardoso ; de Almeida, Luciana Previato ; Ferreira de Miranda Santos, Isabel Kinney ; Silva, Celio Lopes

• Document type: Journal article
• Source: TUBERCULOSIS; v. 101, p. 151-159, DEC 2016.
• Web of Science Citations: 3

Abstract

Tuberculosis (TB) is a major public health concern worldwide; however the factors that account for resistance or susceptibility to disease are not completely understood. Although some studies suggest that the differential expression of miRNAs in peripheral blood of TB patients could be useful as biomarkers of active disease, their involvement during the inflammatory process in lungs of infected individuals is unknown. Here, we evaluated the global expression of miRNAs in the lungs of mice experimentally infected with Mycobacterium tuberculosis on 30 and 60 days post-infection. We observed that several miRNAs were differentially expressed compared to uninfected mice. Furthermore, we verified that the expression of miR-135b, miR-21, miR-155, miR-146a, and miR-146b was significantly altered in distinct leukocyte subsets isolated from lungs of infected mice, while genes potentially targeted by those miRNAs were associated with a diversity of immune related molecular pathways. Importantly, we validated the inhibition of Pellino 1 expression by miR-135b in vitro. Overall, this study contributes to the understanding of the dynamics of miRNA expression in lungs during experimental TB and adds further perspectives into the role of miRNAs on the regulation of immune processes such as leukocyte activation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/25856-8 - The Role of IgG Fc N-Glycosylation on the Pathogenesis of Visceral Leishmaniasis: Insights Into New Strategies of Therapy
• Grantee: Luiz Gustavo Araujo Gardinassi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/04405-8 - TRYPTOPHAN METABOLISM AND RESISTANCE TO TICK INFESTATIONS
• Grantee: Thiago Malardo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/01117-3 - MirRNA analysis of dendritic cells from lung lymph nodes stimulated with mRNAhsp65 or DNAhsp65 from mice infected with experimental tuberculosis
• Grantee: Arlete Aparecida Martins Coelho-Castelo
• Support Opportunities: Regular Research Grants