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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pharmacological Beta-Adrenergic Receptor Activation Attenuates Neutrophil Recruitment by a Mechanism Dependent on Nicotinic Receptor and the Spleen

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Author(s):
Silva, Rangel L. ; Castanheira, Fernanda V. ; Figueiredo, Jozi G. ; Bassi, Gabriel S. ; Ferreira, Sergio H. ; Cunha, Fernando Q. ; Cunha, Thiago M. ; Kanashiro, Alexandre
Total Authors: 8
Document type: Journal article
Source: Inflammation; v. 39, n. 4, p. 1405-1413, AUG 2016.
Web of Science Citations: 2
Abstract

The aim of this study was to identify the effect of beta-adrenergic receptor activation on neutrophil migration in experimental peritonitis elucidating the neuroimmune components involved such as nicotinic receptors and the spleen. Mice pre-treated with mecamylamine (nicotinic antagonist) and propranolol (beta-adrenergic antagonist) or splenectomized animals were treated with isoproterenol (beta-adrenergic agonist) prior to intraperitoneal injection of carrageenan. After 4 h, the infiltrating neutrophils and the local cytokine/chemokine levels were evaluated in the peritoneal lavage. The effect of isoproterenol on neutrophil chemotaxis was investigated in a Boyden chamber. Isoproterenol inhibited neutrophil trafficking, reducing the cytokine/chemokine release and neutrophil chemotaxis. Surprisingly, the isoproterenol effect on neutrophil migration was totally reverted by splenectomy and mecamylamine pre-treatment. In contrast, the inhibitory effect of nicotine on neutrophil migration was abrogated only by splenectomy but not by propranolol pre-treatment. Collectively, our data show that beta-adrenergic receptor activation regulates the acute neutrophil recruitment via splenic nicotinic receptor. (AU)

FAPESP's process: 11/20343-4 - Antiinflammatory cholinergic pathway: the role of neuroimmunomodulation in the control of inflammatory response
Grantee:Alexandre Kanashiro
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/01466-3 - The role of cholinergic anti-inflammatory pathway in the neutrophil migration in model of experimental peritonitis
Grantee:Matheus Christovam Mangetti
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis
Grantee:Fernando de Queiroz Cunha
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
Grantee:Fernando de Queiroz Cunha
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/04237-2 - Antiinflammatory cholinergic pathway: the role of neuroimmunomodulation in the control of inflammatory response
Grantee:Alexandre Kanashiro
Support type: Scholarships in Brazil - Young Researchers