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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oleic acid surfactant in polycaprolactone/hydroxyapatite-composites for bone tissue engineering

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Author(s):
Cardoso, Guinea B. C. ; Maniglio, Devid ; Volpato, Fabio Z. ; Tondon, Abhishek ; Migliaresi, Claudio ; Kaunas, Roland R. ; Zavaglia, Cecilia A. C.
Total Authors: 7
Document type: Journal article
Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 104, n. 6, p. 1076-1082, AUG 2016.
Web of Science Citations: 7
Abstract

Bone substitutes are required to repair osseous defects caused by a number of factors, such as traumas, degenerative diseases, and cancer. Autologous bone grafting is typically used to bridge bone defects, but suffers from chronic pain at the donor-site and limited availability of graft material. Tissue engineering approaches are being investigated as viable alternatives, which ideal scaffold should be biocompatible, biodegradable, and promote cellular interactions and tissue development, need to present proper mechanical and physical properties. In this study, poly(epsilon-caprolactone) (PCL), oleic acid (OA) and hydroxyapatite (HAp) were used to obtain films whose properties were investigated by contact angle, scanning electron microscopy, atomic force microscopy, tensile mechanical tests, and in vitro tests with U2OS human osteosarcoma cells by direct contact. Our results indicate that by using OA as surfactant/dispersant, it was possible to obtain a homogenous film with HAp. The PCL/OA/Hap sample had twice the roughness of the control (PCL) and a lower contact angle, indicating increased hydrophilicity of the film. Furthermore, mechanical testing showed that the addition of HAp decreased the load at yield point and tensile strength and increased tensile modulus, indicating a more brittle composition vs. PCL matrix. Preliminary cell culture experiments carried out with the films demonstrated that U2OS cells adhered and proliferated on all surfaces. The data demonstrate the improved dispersion of HAp using OA and the important consequences of this addition on the composite, unveiling the potentially of this composition for bone growth support. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1076-1082, 2016. (AU)

FAPESP's process: 14/08625-2 - Detailed characterization of the hybrid biomaterial chitosan/polycaprolactone
Grantee:Guinea Brasil Camargo Cardoso
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 13/19472-0 - Investigation of hybrid biomaterial chitosan/polycaprolactone intraperitoneal barrier; focus on controlled release chemotherapy
Grantee:Guinea Brasil Camargo Cardoso
Support type: Scholarships in Brazil - Post-Doctorate