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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Two-step cleanup procedure for the identification of carotenoid esters by liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry

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Rodrigues, Daniele Bobrowski ; Barros Mariutti, Lilian Regina ; Mercadante, Adriana Zerlotti
Total Authors: 3
Document type: Journal article
Source: Journal of Chromatography A; v. 1457, p. 116-124, JUL 29 2016.
Web of Science Citations: 10

Carotenoids are naturally found in both free form and esterified with fatty acids in most fruits; however, up to now the great majority of studies only evaluated their composition after saponification. This fact is easily explained by the difficult to analyze carotenoid esters. Preliminary studies showed that cleanup procedures in the extract are necessary for further analysis by LC-MS/MS since triacylglycerols (TAGs) impair the MS detection. Considering these facts, we developed a new cleanup procedure to remove TAGs and other lipids from carotenoid fruit extracts. This procedure is based on physical removal of solid lipids at low temperature followed by open column chromatography on MgO and diatomaceous earth. Before cleanup, four carotenoid diesters and two free xanthophylls were identified in murici (Byrsonyma crassifolia), corresponding to about 65% of the total chromatogram area. After carrying out the two-step cleanup procedure, 35 carotenoids were identified, being 14 monoesters, six free carotenoids and 15 carotenoid diesters. We can conclude that this two-step procedure was successfully applied to murici, an Amazonian fruit, which contains high amounts of lipids. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/20988-8 - In vitro bioaccessibility of carotenoids and phenolic compounds in emulsions: compounds formed and influence on the antioxidant capacity
Grantee:Adriana Zerlotti Mercadante
Support type: Regular Research Grants
FAPESP's process: 12/01506-2 - In vitro bioaccessibility of carotenoids and phenolic compounds from Byrsonima crassifolia in food mimic systems: evaluation of the chemical compounds formed and influence on the scavenging capacity against reactive species
Grantee:Lilian Regina Barros Mariutti
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/23218-1 - In vitro bioaccessibility of murici carotenoids and their esters and influence of phenolic compounds on carotenoid bioaccessibility in mimic food system
Grantee:Daniele Bobrowski Rodrigues
Support type: Scholarships in Brazil - Doctorate