| Full text | |
| Author(s): |
Siqueira, Adriane S.
;
Pinto, Monique P.
;
Cruz, Mario C.
;
Smuczek, Basilio
;
Cruz, Karen S. P.
;
Barbuto, Jose Alexandre M.
;
Hoshino, Daisuke
;
Weaver, Alissa M.
;
Freitas, Vanessa M.
;
Jaeger, Ruy G.
Total Authors: 10
|
| Document type: | Journal article |
| Source: | ONCOTARGET; v. 7, n. 30, p. 47904-47917, JUL 26 2016. |
| Web of Science Citations: | 7 |
| Abstract | |
Laminin peptides influence tumor behavior. In this study, we addressed whether laminin peptide C16 (KAFDITYVRLKF, gamma 1 chain) would increase invadopodia activity of cells from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). We found that C16 stimulates invadopodia activity over time in both cell lines. Rhodamine-conjugated C16 decorates the edge of cells, suggesting a possible binding to membrane receptors. Flow cytometry showed that C16 increases activated beta 1 integrin, and beta 1 integrin miRNA-mediated depletion diminishes C16-induced invadopodia activity in both cell lines. C16 stimulates Src and ERK 1/2 phosphorylation, and ERK 1/2 inhibition decreases peptide-induced invadopodia activity. C16 also increases cortactin phosphorylation in both cells lines. Based on our findings, we propose that C16 regulates invadopodia activity over time of squamous carcinoma and fibrosarcoma cells, probably through beta 1 integrin, Src and ERK 1/2 signaling pathways. (AU) | |
| FAPESP's process: | 09/17336-6 - Laminin peptides inducing invadopodia in a cell line derived from squamous cell carcinoma: dynamic study by 4D microscopy |
| Grantee: | Adriane Sousa de Siqueira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |