Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular and serological characterization of the first Leptospira santarosai strain isolated from a dog

Full text
Author(s):
Miotto, Bruno Alonso ; Moreno, Luisa Zanolli ; Alves Guilloux, Aline Gil ; de Sousa, Gisele Oliveira ; Loureiro, Ana Paula ; Moreno, Andrea Micke ; Lilenbaum, Walter ; Vasconcellos, Silvio Arruda ; Heinemann, Marcos Bryan ; Hagiwara, Mitika Kuribayashi
Total Authors: 10
Document type: Journal article
Source: Acta Tropica; v. 162, p. 1-4, OCT 2016.
Web of Science Citations: 6
Abstract

Leptospirosis is a zoonotic disease of global importance caused by pathogenic Leptospira species. Dogs can become asymptomatically infected, acting like reservoir hosts for pathogenic Leptospira, notably Leptospira interrogans serovar Canicola. Identification of such individuals and characterization of leptospires involved in chronic infections may unravel the role of dogs in the epidemiology of particular leptospiral strains. The aim of the present work was to describe the first Leptospira santarosai strain isolated from a dog. The dog was kept in a public shelter in Sao Paulo city, Brazil, and presented asymptomatic urinary shedding detected by PCR. Prospective evaluation was performed to fully characterize its chronic carrier state. The dog did not present anti-Leptospira titles or clinical/laboratorial abnormalities during the evaluations; nevertheless long-term urinary shedding was confirmed by PCR and leptospires were recovered from two occasions. The isolated strain was molecularly characterized by partial 16S rRNA and secY gene sequencing and MLST analysis. Serogroup identification was performed using polyclonal antibodies. The strain was identified as Leptospira santarosai, serogroup Sejroe. This is the first evidence in the literature of the isolation of L santarosai in dogs. Our findings show that dogs can persistently harbor leptospires other than L interrogans. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/14681-7 - Evaluation of urinary shedding and identification of chronic carriers of pathogenic leptospiras in dogs holded at public or private animal shelters of São Paulo Metroplitan area
Grantee:Mitika Kuribayashi Hagiwara
Support type: Regular Research Grants
FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by Pulsed Field Gel Electrophoresis (PFGE) typing and comparative genomic analysis
Grantee:Luisa Zanolli Moreno
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/13022-0 - Study of urinary shedding and identification of chronic carriers of pathogenic leptospiras in dogs kept in public or private animal shelters of Metropolitan São Paulo area
Grantee:Bruno Alonso Miotto
Support type: Scholarships in Brazil - Master