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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigating possible biological targets of Bj-CRP, the first cysteine-rich secretory protein (CRISP) isolated from Bothrops jararaca snake venom

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Lodovicho, Marina E. ; Costa, Tassia R. ; Bernardes, Carolina P. ; Menaldo, Danilo L. ; Zoccal, Karina F. ; Carone, Sante E. ; Rosa, Jose C. ; Pucca, Manuela B. ; Cerni, Felipe A. ; Arantes, Eliane C. ; Tytgat, Jan ; Faccioli, Lucia H. ; Pereira-Crott, Luciana S. ; Sampaio, Suely V.
Total Authors: 14
Document type: Journal article
Source: Toxicology Letters; v. 265, p. 156-169, JAN 4 2017.
Web of Science Citations: 9
Abstract

Cysteine-rich secretory proteins (CRISPs) are commonly described as part of the protein content of snake venoms, nevertheless, so far, little is known about their biological targets and functions. Our study describes the isolation and characterization of Bj-CRP, the first CRISP isolated from Bothrops jararaca snake venom, also aiming at the identification of possible targets for its actions. Bj-CRP was purified using three chromatographic steps (Sephacryl S-200, Source 15Q and C18) and showed to be an acidic protein of 24.6 kDa with high sequence identity to other snake venom CRISPs. This CRISP was devoid of proteolytic, hemorrhagic or coagulant activities, and it did not affect the currents from 13 voltage-gated potassium channel isoforms. Conversely, Bj-CRP induced inflammatory responses characterized by increase of leukocytes, mainly neutrophils, after 1 and 4 h of its injection in the peritoneal cavity of mice, also stimulating the production of IL-6. Bj-CRP also acted on the human complement system, modulating some of the activation pathways and acting directly on important components (C3 and C4), thus inducing the generation of anaphylatoxins (C3a, C4a and C5a). Therefore, our results for Bj-CRP open up prospects for better understanding this class of toxins and its biological actions. (C) 2016 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/12954-6 - Study of the toxins Ts6 e Ts15 from the scorpion Tityus serrulatus as potential therapeutic drugs for the treatment of autoimmune diseases
Grantee:Manuela Berto Pucca
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/13590-8 - Isolation, molecular and functional characterization of a new toxin from Tityus serrulatus scorpion venom
Grantee:Felipe Augusto Cerni
Support type: Scholarships in Brazil - Doctorate