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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture

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Author(s):
Valdetaro, Gisele P. ; Aldrovani, Marcela ; Padua, Ivan R. M. ; Cristovam, Priscila C. ; Gomes, Jose A. P. ; Laus, Jose L.
Total Authors: 6
Document type: Journal article
Source: BIOMEDICAL OPTICS EXPRESS; v. 7, n. 12, p. 4982-4994, DEC 1 2016.
Web of Science Citations: 2
Abstract

In this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure-induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. (C)2016 Optical Society of America (AU)

FAPESP's process: 13/25533-1 - MICROENVIRONMENTAL REGULATION OF CORNEAL LIMBAL EPITHELIAL CELLS CULTURED ON DENUDED AMNIOTIC MEMBRANE: EMPHASIS IN SUPRAMOLECULAR ORGANIZATION OF MAJOR EXTRACELLULAR BIOPOLYMERS
Grantee:Gisele Pereira Valdetaro
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/17308-5 - Chromatin supraorganization, DNA content, nuclear glycoprotein amount, immunophenotypic characterization of corneal limbal epithelial cells cultivated on intact or denuded amniotic membrane versus scaffold composed of fucoidan-alginate-chitosan
Grantee:Marcela Aldrovani Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/01494-7 - Chromatin supraorganization, DNA content, nuclear glycoprotein amount, and immunophenotypic characterization of limbal epithelial cells cultured on intact or denuded amniotic membrane versus scaffold composed of fucoidan-alginate-chitosan
Grantee:José Luiz Laus
Support Opportunities: Regular Research Grants