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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi Fusarium oxysporum, Fusarium moniliforme and Fusarium solani

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Author(s):
de Menezes, Henrique Dantas ; Tonani, Ludmilla ; Bachmann, Luciano ; Wainwright, Mark ; Leite Braga, Gilberto Ubida ; von Zeska Kress, Marcia Regina
Total Authors: 6
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 164, p. 1-12, NOV 2016.
Web of Science Citations: 6
Abstract

The search for alternatives to control microorganisms is necessary both in clinical and agricultural areas. Antimicrobial photodynamic treatment (APDT) is a promising light-based approach that can be used to control both human and plant pathogenic fungi. In the present study, we evaluated the effects of photodynamic treatment with red light and four phenothiazinium photosensitizers (PS): methylene blue (MB), toluidine blue O (TBO), new methylene blue N (NMBN) and the phenothiazinium derivative 5137 on ungerminated and germinated microconidia of Fusarium oxysporum, F. moniliforme, and F. solani. APDT with each PS killed efficiently both the quiescent ungerminated microconidia and metabolically active germinated microconidia of the three Fusarium species. Washing away the unbound PS from the microconidia (both ungerminated and germinated) before red light exposure reduced but did not prevent the effect of APDT. Subcelullar localization of PS in ungerminated and germinated microconidia and the effects of photodynamic treatment on cell membranes were also evaluated in the three Fusarium species. APDT with MB, TBO, NMBN or 5137 increased the membrane permeability in microconidia and APDT with NMBN or 5137 increased the lipids peroxidation in microconidia of the three Fusarium species. These findings expand the understanding of photodynamic inactivation of filamentous fungi with phenothiazinium PS. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 15/05853-7 - Effect of antimicrobial photodynamic inactivation in Fonsecaea pedrosoi and different species of Fusarium sp. by using phenothiazine photosensitizers
Grantee:Marcia Regina von Zeska Kress
Support Opportunities: Regular Research Grants
FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants