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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry

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Author(s):
Odir Rodrigues Melo, Carlos Fernando ; Esteves, Cibele Zanardi ; de Oliveira, Rosimeire Nunes ; Guerreiro, Tatiane Melina ; de Oliveira, Diogo Noin ; Lima, Estela de Oliveira ; Mine, Julio Cesar ; Allegretti, Silmara Marques ; Catharino, Rodrigo Ramos
Total Authors: 9
Document type: Journal article
Source: Parasitology Research; v. 115, n. 11, p. 4107-4114, NOV 2016.
Web of Science Citations: 1
Abstract

Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed to identify marker molecules from A. lumbricoides in different development stages. We have identified nine biomarkers, such as pheromones and steroidal prohormones in early stages, among other molecules in late development stages, making up four molecules for fertilized eggs, four marker molecules for first larvae (L1) and one marker molecule for third larvae (L3). Therefore, our findings indicate that this approach is suitable for biochemical characterization of A. lumbricoides development stages. Moreover, the straightforward analytical method employed, with almost no sample preparation from a complex matrix (feces) using high-resolution mass spectrometry, suggests that it is possible to seek for an easier and faster way to study animal molding processes. (AU)

FAPESP's process: 14/00084-2 - Identification of adulterations and simultaneous determination of nutrients and contaminants in food using direct analysis mass spectrometry and imaging
Grantee:Diogo Noin de Oliveira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/06809-1 - Determination of chemical markers in various bovine products using mass spectrometry
Grantee:Rodrigo Ramos Catharino
Support type: Regular Research Grants
FAPESP's process: 14/00302-0 - Lipidomics shotgun in giardiasis, meningitis and tuberculosis: the search of diagnostic markers, using revolutionary new techniques of mass spectrometry
Grantee:Cibele Zanardi Esteves
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
Grantee:Aníbal Eugênio Vercesi
Support type: Research Projects - Thematic Grants