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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A GENOMIC APPROACH FOR ISOLATING CHLOROPLAST MICROSATELLITE MARKERS FOR PACHYPTERA KERERE (BIGNONIACEAE)

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Author(s):
Francisco, Jessica N. C. ; Nazareno, Alison G. ; Lohmann, Lucia G.
Total Authors: 3
Document type: Journal article
Source: APPLICATIONS IN PLANT SCIENCES; v. 4, n. 9 SEP 2016.
Web of Science Citations: 1
Abstract

Premise of the study: In this study, we developed chloroplast microsatellite markers (cpSSRs) for Pachyptera kerere (Bignoniaceae) to investigate the population structure and genetic diversity of this species. Methods and Results: We used Illumina HiSeq data to reconstruct the chloroplast genome of P. kerere by a combination of de novo and reference-guided assembly. We then used the chloroplast genome to develop a set of cpSSRs from intergenic regions. Overall, 24 primer pairs were designed, 21 of which amplified successfully and were polymorphic, presenting three to nine alleles per locus. The unbiased haploid diversity per locus varied from 0.207 (Pac28) to 0.817 (Pac04). All but one locus amplified for all other taxa of Pachyptera. Conclusions: The markers reported here will serve as a basis for studies to assess the genetic structure and phylogeographic history of Pachyptera. (AU)

FAPESP's process: 12/50260-6 - Structure and evolution of the Amazonian biota and its environment: an integrative approach
Grantee:Lúcia Garcez Lohmann
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 11/50859-2 - Systematics of the tribe Bignonieae (Bignoniaceae)
Grantee:Lúcia Garcez Lohmann
Support type: Regular Research Grants
FAPESP's process: 13/12633-8 - Comparative phylogeography of plants in the Central Amazonia
Grantee:Alison Gonçalves Nazareno
Support type: Scholarships in Brazil - Post-Doctorate