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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crystal structure of an Fe-S cluster-containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold

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Author(s):
Feliciano, Patricia R. ; Drennan, Catherine L. ; Cristina Nonato, M.
Total Authors: 3
Document type: Journal article
Source: Proceedings of the National Academy of Sciences of the United States of America; v. 113, n. 35, p. 9804-9809, AUG 30 2016.
Web of Science Citations: 6
Abstract

Fumarate hydratases (FHs) are essential metabolic enzymes grouped into two classes. Here, we present the crystal structure of a class I FH, the cytosolic FH from Leishmania major, which reveals a previously undiscovered protein fold that coordinates a catalytically essential {[}4Fe-4S] cluster. Our 2.05 angstrom resolution data further reveal a dimeric architecture for this FH that resembles a heart, with each lobe comprised of two domains that are arranged around the active site. Besides the active site, where the substrate S-malate is bound bidentate to the unique iron of the {[}4Fe-4S] cluster, other binding pockets are found near the dimeric enzyme interface, some of which are occupied bymalonate, shown here to be a weak inhibitor of this enzyme. Taken together, these data provide a framework both for investigations of the class I FH catalytic mechanism and for drug design aimed at fighting neglected tropical diseases. (AU)

FAPESP's process: 09/10454-3 - Studies of the correlation between structure and function of the enzyme fumarate hydratase in Leishmania major
Grantee:Patrícia Rosa Feliciano
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/19674-6 - Structure determination of fumarate hydratase isoforms from Leishmania major
Grantee:Patrícia Rosa Feliciano
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 08/08262-6 - Kinetic, Structural and functional characterization of LmjF24.0320. e LmjF29.1960 genes that code for fumarate hydratase in Leishmania major
Grantee:Maria Cristina Nonato
Support type: Regular Research Grants