| Full text | |
| Author(s): |
Guimaraes, F. F.
;
Manzi, M. P.
;
Joaquim, S. F.
;
Richini-Pereira, V. B.
;
Langoni, H.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | JOURNAL OF DAIRY SCIENCE; v. 100, n. 1, p. 726-730, JAN 2017. |
| Web of Science Citations: | 12 |
| Abstract | |
Cows are probably the main source of contamination of raw milk with Staphylococcus aureus. Mammary glands with subclinical mastitis can shed large numbers of Staph. aureus in milk. Because of the risk of this pathogen to human health as well as animal health, the aim of this paper was to describe an outbreak of mastitis caused by methicillin-resistant Staph. aureus (MRSA), oxacillin-susceptible mecA-positive Staph. aureus (OS-MRSA), and methicillin-susceptible Staph. aureus (MSSA) on a dairy farm. Milk samples were obtained from all quarters, showing an elevated somatic cell count by the California Mastitis Test. The isolates were identified by phenotypic and genotypic methods. Staphylococcus spp. were isolated from 53% (61/115) of the milk samples, with 60 isolates identified as Staph. aureus (98.4%) and 1 isolate identified as Staphylococcus epidermidis (1.6%). The presence of the mecA gene was verified in 48.3% of Staph. aureus isolates. Of the Staph. aureus isolates, 23.3% were MRSA and 25.0% were OS-MRSA. The total of mastitis cases infected with MRSA was 12.2%. The detection of this large percentage of mastitis cases caused by MRSA and OS-MRSA is of great concern for the animals' health, because 13-lactams are still the most important antimicrobials used to treat mastitis. In addition, Staph. aureus isolates causing bovine mastitis represent a public health risk. (AU) | |
| FAPESP's process: | 08/11614-1 - ROLE OF STAPHYLOCOCCUS COAGULASE NEGATIVE IN BOVINE MASTITIS: MICROBIAL SENSITIVITY PROFILE, RESEARCH OF GENE mecA OF RESISTENCE TO METHICILIN AND MOCELULAR DETECTION OF GENES CODING ENTEROTOXINS |
| Grantee: | Helio Langoni |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/21323-7 - Research of enconding genes of staphylococcal enterotoxins and vancomycin resistance gene vanA: detection of genic expression in Staphylococcus coagulase positive and negative in bovine mastitis |
| Grantee: | Helio Langoni |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 08/08780-7 - Role of coagulase-negative staphylococcus in bovine mastitis: microbial sensitivity profile, research of mecA gene of resistance to meticilina and molecular detection of enterotoxins coding-genes |
| Grantee: | Felipe de Freitas Guimarães |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/21142-2 - Research of encoding genes of staphylococcal enterotoxins and vancomycin resistance gene vanA: detection of genic expression in Staphylococcus coagulase positive and negative in bovine mastitis |
| Grantee: | Felipe de Freitas Guimarães |
| Support Opportunities: | Scholarships in Brazil - Doctorate |