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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of misexpressed genetic elements in hybrids between Drosophila-related species

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Author(s):
Lopez-Maestre, Helene ; Carnelossi, Elias A. G. ; Lacroix, Vincent ; Burlet, Nelly ; Mugat, Bruno ; Chambeyron, Severine ; Carareto, Claudia M. A. ; Vieira, Cristina
Total Authors: 8
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, JAN 16 2017.
Web of Science Citations: 3
Abstract

Crosses between close species can lead to genomic disorders, often considered to be the cause of hybrid incompatibility, one of the initial steps in the speciation process. How these incompatibilities are established and what are their causes remain unclear. To understand the initiation of hybrid incompatibility, we performed reciprocal crosses between two species of Drosophila (D. mojavensis and D. arizonae) that diverged less than 1 Mya. We performed a genome-wide transcriptomic analysis on ovaries from parental lines and on hybrids from reciprocal crosses. Using an innovative procedure of co-assembling transcriptomes, we show that parental lines differ in the expression of their genes and transposable elements. Reciprocal hybrids presented specific gene categories and few transposable element families misexpressed relative to the parental lines. Because TEs are mainly silenced by piwi-interacting RNAs (piRNAs), we hypothesize that in hybrids the deregulation of specific TE families is due to the absence of such small RNAs. Small RNA sequencing confirmed our hypothesis and we therefore propose that TEs can indeed be major players of genome differentiation and be implicated in the first steps of genomic incompatibilities through small RNA regulation. (AU)

FAPESP's process: 10/10731-4 - Evolutionary dynamics and transposable elements regulation in Drosophila populations and species
Grantee:Claudia Marcia Aparecida Carareto
Support type: Regular Research Grants