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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Highly sensitive dual mode electrochemical platform for microRNA detection

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Author(s):
Jolly, Pawan ; Batistuti, Marina R. ; Miodek, Anna ; Zhurauski, Pavel ; Mulato, Marcelo ; Lindsay, Mark A. ; Estrela, Pedro
Total Authors: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 6, NOV 8 2016.
Web of Science Citations: 19
Abstract

MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms. (AU)

FAPESP's process: 15/14403-5 - Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers
Grantee:Marina Ribeiro Batistuti
Support type: Scholarships abroad - Research Internship - Doctorate