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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effects of photodynamic treatment with new methylene blue N on the Candida albicans proteome

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Author(s):
Pereira Brancini, Guilherme Thomaz ; Rodrigues, Gabriela Braga ; Lima Rambaldi, Mariana de Souza ; Izumi, Clarice ; Yatsuda, Ana Patricia ; Wainwright, Mark ; Rosa, Jose Cesar ; Leite Braga, Gilberto Ubida
Total Authors: 8
Document type: Journal article
Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 15, n. 12, p. 1503-1513, 2016.
Web of Science Citations: 8
Abstract

Candida albicans is a human pathogenic fungus mainly affecting immunocompromised patients. Resistance to the commonly used fungicides can lead to poor treatment of mucosal infections which, in turn, can result in life-threatening systemic candidiasis. In this scenario, antimicrobial photodynamic treatment (PDT) has emerged as an effective alternative to treat superficial and localized fungal infections. Microbial death in PDT is a consequence of the oxidation of many cellular biomolecules, including proteins. Here, we report a combination of two-dimensional electrophoresis and tandem mass spectrometry to study the protein damage resulting from treating C. albicans with PDT with new methylene blue N and red light. Two-dimensional gels of treated cells showed an increase in acidic spots in a fluence-dependent manner. Amino acid analysis revealed a decrease in the histidine content after PDT, which is one plausible explanation for the observed acidic shift. However, some protein spots remained unchanged. Protein identification by mass spectrometry revealed that both modified and unmodified proteins could be localized to the cytoplasm, ruling out subcellular location as the only explanation for damage selectivity. Therefore, we hypothesize that protein modification by PDT is a consequence of both photosensitizer binding affinity and the degree of exposure of the photooxidizable residues on the protein surface. (AU)

FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants
FAPESP's process: 13/02486-8 - A proteomic approach for the identification of proteins related to stress tolerance in conidia of the entomopathogenic fungus Metarhizium acridum
Grantee:Guilherme Thomaz Pereira Brancini
Support Opportunities: Scholarships in Brazil - Master