Nanoemulsions encapsulating oregano essential oil: Production, stability, antibacterial activity and incorporation in chicken pate

Author(s)

Moraes-Lovison, Marilia ; Marostegan, Luis F. P. ; Peres, Marina S. ; Menezes, Isabela F. ; Ghiraldi, Marluci ; Rodrigues, Rodney A. F. ; Fernandes, Andrezza M. ; Pinho, Samantha C.

• Document type: Journal article
• Source: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 77, p. 233-240, APR 2017.
• Web of Science Citations: 28

Abstract

Several essential oils (EO) are efficient inhibitors of foodborne pathogen growth. However, their direct incorporation into food presents technological challenges, because of the possibility of extreme changes to their sensory properties. Such drawbacks can be overcome by encapsulation techniques, such as nanoemulsification. This study produced nanoemulsions encapsulating different amounts of oregano (Origanum vulgare) EO, using the phase inversion temperature method, and their physicochemical stability and antibacterial activity were evaluated. The average droplet diameters of the nanoemulsions were in the range of 35-55 nm, and nanoemulsions containing 5 g EO/100 g nanoemulsion were the most stable over the storage period of 45 days. The nanoemulsions exhibited good antibacterial action demonstrated by the low values in vitro for minimum inhibitory concentration (0.56 and 0.60 mg oregano EO/mL for Staphylococcus aureus and Escherichia coli, respectively, and for minimum bactericidal concentration, 0.90 and 3.32 mg oregano EO/mL for Staphylococcus aureus and Escherichia coli, respectively). The nanodispersions also had a higher antibacterial effect against E. coli than for S. aureus. The incorporation of nanoemulsions in chicken pate did not change the physicochemical characteristics of the meat product. Therefore, the results obtained indicated that nanoemulsions encapsulating oregano EO are suitable to be incorporated into food formulations, such as chicken pate, to prevent and control microbial growth and to extend their shelf life. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 13/25182-4 - Oregano essential oil in nanoemulsions: production, physico-chemical characterization, in vitro antimicrobial activity and case study in chicken paté
• Grantee: Marília Moraes Lovison
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 12/01460-2 - Encapsulation of essential oil of Origanum vulgare in lipid nanostructured systems: production, characterization, shelf-life and antimicrobial activity
• Grantee: Samantha Cristina de Pinho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/14443-6 - Nanostructured lipid systems (liposomes and nanoemulsions) encapsulating oregano (Origanum vulgare) essential oil : production, characterization and antimicrobial acition
• Grantee: Marília Moraes Lovison
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/20916-4 - Production and characterization of nanoemulsions encapsulating essential oil of Origanum vulgare
• Grantee: Luis Fernando Paulatti Marostegan
• Support Opportunities: Scholarships in Brazil - Scientific Initiation