Gsk3 beta and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease

Author(s)

Teixeira, Felipe Roberti ; Randle, Suzanne J. ; Patel, Shachi P. ; Mevissen, Tycho E. T. ; Zenkeviciute, Grasilda ; Koide, Tie ; Komander, David ; Laman, Heike

• Document type: Journal article
• Source: Biochemical Journal; v. 473, n. 20, p. 3563-3580, OCT 2016.
• Web of Science Citations: 6

Abstract

Fbxo7 is a clinically relevant F-box protein, associated with both cancer and Parkinson's disease (PD). Additionally, SNPs within FBXO7 are correlated with alterations in red blood cell parameters. Point mutations within FBXO7 map within specific functional domains, including near its F-box domain and its substrate recruiting domains, suggesting that deficiencies in SCFFbxo7/PARK15 ubiquitin ligase activity are mechanistically linked to early-onset PD. To date, relatively few substrates of the ligase have been identified. These include HURP (hepatoma up-regulated protein), whose ubiquitination results in proteasome-mediated degradation, and c-IAP1 (inhibitor of apoptosis protein 1), TNF receptor-associated factor 2 (TRAF2), and NRAGE, which are not destabilized as a result of ubiquitination. None of these substrates have been linked directly to PD, nor has it been determined whether they would directly engage neuronal cell death pathways. To discover ubiquitinated substrates of SCFFbxo7 implicated more directly in PD aetiology, we conducted a high-throughput screen using protein arrays to identify new candidates. A total of 338 new targets were identified and from these we validated glycogen synthase kinase 3 beta (Gsk3 beta), which can phosphorylate a-synuclein, and translocase of outer mitochondrial membrane 20 (Tomm20), a mitochondrial translocase that, when ubiquitinated, promotes mitophagy, as SCFFbxo7 substrates both in vitro and in vivo. Ubiquitin chain restriction analyses revealed that Fbxo7 modified Gsk3 beta using K63 linkages. Our results indicate that Fbxo7 negatively regulates Gsk3 beta activity, rather than its levels or localization. In addition, Fbxo7 ubiquitinated Tomm20, and its levels correlated with Fbxo7 expression, indicating a stabilizing effect. None of the PD-associated mutations in Fbxo7 impaired Tomm20 ubiquitination. Our findings demonstrate that SCFFbxo7 has an impact directly on two proteins implicated in pathological processes leading to PD. (AU)

• FAPESP's process: 10/16464-8 - Application of protein microarray to identify substrates of SCF1 (FBXO25) E3 ubiquitin ligase
• Grantee: Felipe Roberti Teixeira
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 12/09241-8 - Identification of the substrates of the E3 ubiquitin-ligase SCF(Fbxo7) using human proteome microarray
• Grantee: Felipe Roberti Teixeira
• Support type: Scholarships abroad - Research Internship - Post-doctor