Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft

Author(s)

Immich, A. P. S. ; Pennacchi, P. C. ; Naves, A. F. ; Felisbino, S. L. ; Boemo, R. L. ; Maria-Engler, S. S. ; Catalani, L. H.

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 73, p. 48-58, APR 1 2017.
• Web of Science Citations: 4

Abstract

Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (L-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30 days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/22812-1 - DEVELOPMENT OF ARTIFICIAL SKIN CONTAINING GLYCATED DERMAL EQUIVALENT MIMICKING THE PATHOPHYSIOLOGY OF SKIN AGING AND DIABETES
• Grantee: Paula Comune Pennacchi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/21442-6 - Synthetic and natural polymers applied to tissue engineering
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/18017-1 - DEVELOPMENT OF ELECTROSPUN POLYMERIC MATRICES FOR THE REGENERATION OF TYMPANIC MEMBRANE PERFORATIONS.
• Grantee: Ana Paula Serafini Immich Boemo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral