| Full text | |
| Author(s): |
Lopes Carapeto, Fernando Cintra
;
Comodo, Andreia Neves
;
Germano, Andressa
;
Guimaraes, Daiane Pereira
;
Barcelos, Denise
;
Fernandes, Mariana
;
Landman, Gilles
Total Authors: 7
|
| Document type: | Journal article |
| Source: | AMERICAN JOURNAL OF DERMATOPATHOLOGY; v. 39, n. 2, p. 114-120, FEB 2017. |
| Web of Science Citations: | 2 |
| Abstract | |
Patients and Methods: Samples of acral lentiginous melanomas (ALMs) were obtained from the Department of Pathology at Escola Paulista de Medicina-Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil. Demographic, clinical, and follow-up data were obtained from the charts of Hospital Sao Paulo. From 2 tissue micro-arrays containing 60 nevi and quadruplicate samples of >= 1.0-mm of 49 ALM, sections were stained to evaluate SCF, KIT, BRAF, CYCLIND1, MYC, and PTEN immunohistochemical protein expression. Results: Nevi and ALM from 2006 to 2010 were reviewed and collected. All specimens were in the vertical growth phase, and histopathological parameters indicated that tumors were at an advanced stage at diagnosis. Average tumor thickness was 6.95 mm, 63% were ulcerated, average mitotic index was 5 mitotic cells per mm(2), and 43% were at Clark's level V. Compared with nevi, the x(2) test showed that ALM significantly correlated with SCF protein expression (P = 0.001) and expression heterogeneity (P, 0.000). Similar findings were observed for KIT (P = 0.005, P = 0.003, respectively), MYC (P, 0.000, P < 0.000), and PTEN (P = 0.005, P, 0.000). Malignancy did not correlate with BRAF and CYCLIN D1 expression (P = 0.053 and P = 0.259, respectively), but it did significantly correlate with their heterogeneous expression (P < 0.000, P = 0.024, respectively). Combined protein expression had an odds ratio of greater malignancy when BRAF and MYC were positive and/or heterogeneously expressed (OR of 78 and 95, respectively). Discussion and Conclusion: We show that marker protein expression, when combined with heterogeneous expression as shown by immunohistochemistry, is a powerful indicator of malignancy in ALMs, especially, when protein pairs are combined. (AU) | |
| FAPESP's process: | 11/20435-6 - Acral lentiginous melanoma: analysis of immunohistochemical expression of PTEN protein, c-kit, Cyclin D1, BRAF, Myc and evaluation of the genes CCND1, PTEN, Myc, CCND1, RREB1 , Myb and centromere 6 by fish technique |
| Grantee: | Gilles Landman |
| Support Opportunities: | Regular Research Grants |