MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

Author(s)

Korvala, Johanna ^[1] ; Mosakhani, Neda ; Zandonadi, Flavia S. ; Meirelles, Gabriela V. ^[2] ; Paes Leme, Adriana Franco ^[10] ; Coletta, Ricardo D. ; Leivo, Ilmo ; Salo, Tuula

Affiliation

^[1] CNPEM, LNBio, Lab Nacl Biociencias, 10-000 Polo II Alta Tecnol Campinas, Campinas S-P, BR-13083970 Campinas, SP, Brazil. Salo, Tuula, Univ Helsinki, Oral & Maxillofacial Dis, Helsinki, Finland. Salo, Tuula, Helsinki Univ Hosp, Helsinki - Finland

^[2] Coletta, Ricardo D., Univ Campinas UNICAMP, Sch Dent, Dept Oral Diag, Av Limeira 901 Bairro Areiao, BR-13414903 Piracicaba, SP, Brazil. Cervigne, Nilva K., FMJ, Dept Clin \& Pathol, Jundiai, SP, Brazil. Zandonadi, Flavia S., Leivo, Ilmo, Univ Helsinki, Ha - Finland

^[3] Coletta, Ricardo D., Univ Campinas UNICAMP, Sch Dent, Dept Oral Diag, Av Limeira 901 Bairro Areiao, BR-13414903 Piracicaba, SP, Brazil. Cervigne, Nilva K., FMJ, Dept Clin \& Pathol, Jundiai, SP, Brazil. Zandonadi, Flavia S., Leivo, Ilmo, Univ Helsinki, Haartman Inst, Dept Pathol, Helsinki, Finland. Mosakhani, Neda, HUSLAB, Dept Pathol, Helsinki, Finland. Cervigne, Nilva K., Leivo, Ilmo, Univ Turku, Turku Univ Hosp, Dept Pathol, Turku, Finland. Jee, Kowan, Salo, Tuula, Univ Oulu, Oulu Univ Hosp, Med Res Ctr Oulu, Canc \& Translat Med Res Unit, Aapistie 5A, Oulu 90014, Finland. Jee, Kowan, CNPEM, LNBio, Lab Nacl Biociencias, 10-000 Polo II Alta Tecnol Campinas, Campinas S-P, BR-13083970 Campinas, SP, Brazil. Salo, Tuula, Univ Helsinki, Oral & Maxillofacial Dis, Helsinki, Finland. Salo, Tuula, Helsinki Univ Hosp, Helsinki - Finland

• Document type: Journal article
• Source: Experimental Cell Research; v. 350, n. 1, p. 9-18, JAN 1 2017.
• Web of Science Citations: 9

Abstract

Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated "Focal adhesion" and "ECM-receptor interaction" as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren't significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15. (AU)

• FAPESP's process: 09/54067-3 - Acquisition of a mass spectrometer coupled to a liquid chromatography system for increasing the capacity to meet the needs of users and for making new technologies available in the Laboratory of Mass Spectrometry
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/01607-6 - The biological role of EEF1D, fascin and plectin on oral squamous cell carcinoma
• Grantee: Ricardo Della Coletta
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/19278-0 - Study of regulation of ADAMs in oral cancer
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Research Grants - Young Investigators Grants