| Full text | |
| Author(s): |
Anovazzi, Giovana
;
de Medeiros, Marcell Costa
;
Pigossi, Suzane Cristina
;
Finoti, Livia Sertori
;
Alves Mayer, Marcia Pinto
;
Rossa Junior, Carlos
;
Scarel-Caminaga, Raquel Mantuaneli
Total Authors: 7
|
| Document type: | Journal article |
| Source: | PLoS One; v. 12, n. 1 JAN 23 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
Chronic periodontitis (CP) is an infectious inflammatory disease that affects tooth-supporting structures and in which dental plaque bacteria, immune mechanisms and genetic predisposition play important roles. Interleukin 4 (IL-4) is a key anti-inflammatory cytokine with relevant action in imbalances in inflamed periodontal tissue. Individuals carrying the TCI/CCI genotype (S-haplotype) of the IL-4 gene are 5 times more susceptible to CP, whereas the CTI/TTD genotype (P-haplotype) confers protection against CP. Compared with the S-haplotype, subjects with the P-haplotype produce higher levels of the IL-4 protein after non-surgical periodontal therapy. The present in vitro study aimed to investigate the functionality of IL-4 haplotypes in immune cells to obtain insight into the influence of these genetic variations in regulating immune responses to CP-associated bacteria. Peripheral blood was collected from 6 subjects carrying each haplotype, and their immune cells were challenged with periodontopathogens to compare responses of the different haplotypes with regard to gene expression, protein secretion and the immunophenotype of T helper responses. We found higher IL-4 mRNA and protein levels in the P-haplotype, which also presented higher levels of anti-inflammatory cytokines. In contrast, cells from S-haplotype subjects responded with higher levels of pro-inflammatory cytokines. S-haplotype individuals exhibited significantly greater polarization toward the Th1 phenotype, whereas the Phaplotype was associated with an attenuated response to periodontopathogens, with suggestive skewing toward Th2/M2 phenotypes. In conclusion, IL-4 genetic variations associated with susceptibility to or protection against chronic periodontitis are directly associated with influencing the response of immune cells to periodontopathogens. (AU) | |
| FAPESP's process: | 14/04638-2 - Investigation of the functional role of haplotypes in the interleukin 4 gene |
| Grantee: | Giovana Anovazzi Medeiros |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/17887-8 - Investigation of the functional role of haplotypes in the interleukin 4 and interleukin 8 genes |
| Grantee: | Raquel Mantuaneli Scarel Caminaga |
| Support Opportunities: | Regular Research Grants |