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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spatial Genetic Structure of Coffee-Associated Xylella fastidiosa Populations Indicates that Cross Infection Does Not Occur with Sympatric Citrus Orchards

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Author(s):
Francisco, Carolina S. ; Ceresini, Paulo C. ; Almeida, Rodrigo P. P. ; Coletta-Filho, Helvecio D.
Total Authors: 4
Document type: Journal article
Source: PHYTOPATHOLOGY; v. 107, n. 4, p. 395-402, APR 2017.
Web of Science Citations: 7
Abstract

Xylella fastidiosa, an economically important plant-pathogenic bacterium, infects both coffee and citrus trees in Brazil. Although X. fastidiosa in citrus is well studied, knowledge about the population structure of this bacterium infecting coffee remains unknown. Here, we studied the population structure of X. fastidiosa infecting coffee trees in Sao Paulo State, Brazil, in four regions where citrus is also widely cultivated. Genotyping of over 500 isolates from coffee plants using 14 genomic microsatellite markers indicated that populations were largely geographically isolated, as previously found with populations of X. fastidiosa infecting citrus. These results were supported by a clustering analysis, which indicated three major genetic groups among the four sampled regions. Overall, approximately 38% of isolates showed significant membership coefficients not related to their original geographical populations (i.e., migrants), characterizing a significant degree of genotype flow among populations. To determine whether admixture occurred between isolates infecting citrus and coffee plants, one site with citrus and coffee orchards adjacent to each other was selected; over 100 isolates were typed from each host plant. No signal of natural admixture between citrus-and coffee-infecting isolates was found; artificial cross-infection assays with representative isolates also yielded no successful cross infection. A comparison determined that X. fastidiosa populations from coffee have higher genetic diversity and allelic richness compared with citrus. The results showed that coffee and citrus X. fastidiosa populations are effectively isolated from each other and, although coffee populations are spatially structured, migration has an important role in shaping diversity. (AU)

FAPESP's process: 11/13803-9 - Population structure and reciprocal inoculation of Xylella fastidiosa subsp. pauca occurring in neighbor orchards of citrus sinensis and Coffea arabica growing in the São Paulo State
Grantee:Helvecio Della Coletta Filho
Support Opportunities: Regular Research Grants
FAPESP's process: 11/14479-0 - Structure of populations and reciprocal inoculations of Xylella fastidiosa subsp. pauca occurring in neighboring cultures of Citrus sinensis and Coffea arabica in conditions of São Paulo.
Grantee:Carolina Sardinha Francisco
Support Opportunities: Scholarships in Brazil - Master