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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The gut microbiota of insecticide-resistant insects houses insecticide-degrading bacteria: A potential source for biotechnological exploitation

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Author(s):
de Almeida, Luis Gustavo ; Beraldo de Moraes, Luiz Alberto ; Trigo, Jose Roberto ; Omoto, Celso ; Consoli, Fernando Luis
Total Authors: 5
Document type: Journal article
Source: PLoS One; v. 12, n. 3 MAR 30 2017.
Web of Science Citations: 23
Abstract

The exploration of new niches for microorganisms capable of degrading recalcitrant molecules is still required. We hypothesized the gut microbiota associated with insect-resistant lines carry pesticide degrading bacteria, and predicted they carry bacteria selected to degrade pesticides they were resistant to. We isolated and accessed the pesticide-degrading capacity of gut bacteria from the gut of fifth instars of Spodoptera frugiperda strains resistant to lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, spinosad and lufenuron, using insecticide-selective media. Sixteen isolates belonging to 10 phylotypes were obtained, from which four were also associated with the susceptible strain. However, growth of gut bacteria associated with larvae from the susceptible strain was not obtained in any of the insecticide-based selective media tested. Growth of isolates was affected by the concentration of insecticides in the media, and all grew well up to 40 lag/ml. The insecticide degrading capacity of selected isolates was assessed by GC or LC-MS/MS analyses. In conclusion, resistant strains of S. frugiperda are an excellent reservoir of insecticide-degrading bacteria with bioremediation potential. Moreover, gut-associated bacteria are subjected to the selection pressure imposed by insecticides on their hosts and may influence the metabolization of pesticides in insects. (AU)

FAPESP's process: 10/13714-3 - Insect symbionts and the degradation of xenobiotics: implications for the development of insect resistance to insecticides and their potential for bioremediation
Grantee:Luis Gustavo de Almeida
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/50877-0 - Diversity, ecology and biotechnological potential of the symbiotic bacteriofauna associated with insects
Grantee:Fernando Luis Cônsoli
Support type: BIOTA-FAPESP Program - Thematic Grants