Antimicrobial activity against Gram-positive and Gram-negative bacteria during gatifloxacin degradation by hydroxyl radicals

Author(s)

Caianelo, Marlon ; Rodrigues-Silva, Caio ; Maniero, Milena Guedes ; Guimaraes, Jose Roberto

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 24, n. 7, p. 6288-6298, MAR 2017.
• Web of Science Citations: 9

Abstract

Gatifloxacin, an antimicrobial drug belonging to the fluoroquinolone family, is active against Gram-positive and Gram-negative bacteria and is extensively used for the control of infections in humans. The presence of the drug in environmental matrices has already been reported. This study investigated the degradation of gatifloxacin in water by hydroxyl radicals generated by the UV254 nm/H2O2 process (CH2O2initial = 0.4-2.4 mmol L-1) and evaluated the capacity of the radicals to reduce the antimicrobial activity against Gram-positive and Gram-negative bacteria. Acute toxicity assays were performed with Vibrio fischeri, and the degradation products were proposed. The hydroxyl radicals formed in the processes were able to degrade the fluoroquinolone and remove the antimicrobial activity from the aqueous solution. Approximately 97 % gatifloxacin degradation was observed after applying 2.4 mmol L-1 of initial H2O2 concentration and 20 min of UVC254nm irradiation (130 J s(-1)). The acute toxicity assays showed that the toxicity of the treated solution for V. fischeri increased as the gatifloxacin concentration in the solution decreased. (AU)

• FAPESP's process: 14/16622-3 - Identification of intermediates formed during degradation of veterinary drugs by advanced oxidation processes
• Grantee: Caio Alexandre Augusto Rodrigues da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/06064-3 - Multi-User Equipment aproved in grant 2013/09543-7: ultra high performance liquid chromatography with photodiode array detector and Fluorescence Detector (UPLC-DAD-FLD)
• Grantee: Susanne Rath
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/09543-7 - Residues of veterinary drugs in the environment
• Grantee: Susanne Rath
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/04656-8 - Degradation of pharmaceuticals by peroxidation combined with ultraviolet radiation and ozonation: antimicrobial activity and toxicity
• Grantee: José Roberto Guimarães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07817-2 - Degradation of sulfonamides and tetraciclines by UV/H2O2 and O3: evaluation of antimicrobial activity and toxicity
• Grantee: Milena Guedes Maniero Ferreira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/06201-0 - Multi-equipment aproved in grant 2013/09543-7: ultra high performance liquid chromatograph coupled to a photodiode array detector and tandem mass spectrometer (UPLC-DAD-MS/MS)
• Grantee: Susanne Rath
• Support Opportunities: Multi-user Equipment Program