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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

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Bella, Leonardo M. ; Fieri, Isis ; Tessaro, Fernando H. G. ; Nolasco, Eduardo L. ; Nunes, Fernanda P. B. ; Ferreira, Sabrina S. ; Azevedo, Carolina B. ; Martins, Joilson O.
Total Authors: 8
Document type: Journal article
Web of Science Citations: 2

Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60mg/kg i.v., 10 days) and non diabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice. (AU)

FAPESP's process: 12/23998-4 - Influence of vitamin D receptor expression (VDR and TLR) in macrophages in the model of experimental diabetes mellitus
Grantee:Leonardo Mendes Bella
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/05214-1 - Investigating the role of insulin in different infections in diabetic and healthy animals
Grantee:Joilson de Oliveira Martins
Support type: Regular Research Grants
FAPESP's process: 13/20904-1 - Study of the expression of Vitamin D receptor in peritoneal and alveolar macrophages in healthy and diabetic mice
Grantee:Isis Fieri
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/02272-0 - Effect of insulin on lung inflammation in animal with sepsis, innate immunit,activation of insulin gene (BGK) and insulin receptors (IR)-A and IR-B
Grantee:Joilson de Oliveira Martins
Support type: Research Grants - Young Investigators Grants