| Full text | |
| Author(s): Show less - |
Kurauti, Mirian A.
;
Costa-Junior, Jose M.
;
Ferreira, Sandra M.
;
Santos, Gustavo J.
;
Sponton, Carlos H. G.
;
Carneiro, Everardo M.
;
Telles, Guilherme D.
;
Chacon-Mikahil, Mara P. T.
;
Cavaglieri, Claudia R.
;
Rezende, Luiz F.
;
Boschero, Antonio C.
Total Authors: 11
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| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 7, APR 21 2017. |
| Web of Science Citations: | 9 |
| Abstract | |
Impairment of the insulin-degrading enzyme (IDE) is associated with obesity and type 2 diabetes mellitus (T2DM). Here, we used 4-mo-old male C57BL/6 interleukin-6 (IL-6) knockout mice (KO) to investigate the role of this cytokine on IDE expression and activity. IL-6 KO mice displayed lower insulin clearance in the liver and skeletal muscle, compared with wild type (WT), due to reduced IDE expression and activity. We also observed that after 3-h incubation, IL-6, 50 and 100 ng ml(-1), increased the expression of IDE in HEPG2 and C2C12 cells, respectively. In addition, during acute exercise, the inhibition of IL-6 prevented an increase in insulin clearance and IDE expression and activity, mainly in the skeletal muscle. Finally, IL-6 and IDE concentrations were significantly increased in plasma from humans, after an acute exercise, compared to pre-exercise values. Although the increase in plasma IDE activity was only marginal, a positive correlation between IL-6 and IDE activity, and between IL-6 and IDE protein expression, was observed. Our outcomes indicate a novel function of IL-6 on the insulin metabolism expanding the possibilities for new potential therapeutic strategies, focused on insulin degradation, for the treatment and/or prevention of diseases related to hyperinsulinemia, such as obesity and T2DM. (AU) | |
| FAPESP's process: | 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass |
| Grantee: | Antonio Carlos Boschiero |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/24719-7 - Effect of physical exercise on the expression of peroxisome proliferator activated receptor gamma (PPARgamma) and Insulin Degrading Enzyme (IDE) in mouse pancreatic islets |
| Grantee: | Mirian Ayumi Kurauti |
| Support Opportunities: | Scholarships in Brazil - Doctorate |