Acetylcholine and ventilation: modulation by cholinergic inputs from Pedunculopont...
| Full text | |
| Author(s): |
Malheiros-Lima, Milene R.
;
Takakura, Ana C.
;
Moreira, Thiago S.
Total Authors: 3
|
| Document type: | Journal article |
| Source: | Neuroscience; v. 351, p. 1-14, MAY 20 2017. |
| Web of Science Citations: | 9 |
| Abstract | |
The stimuli that commonly activate the cate-cholaminergic C1 neurons (nociception, hypotension, and hypoxia) also increase breathing. Pharmacogenetic evidence suggests that catecholaminergic neurons regulate breathing. Therefore, we evaluated whether the loss of C1 cells affects cardiorespiratory control during resting, hypoxic (8% O-2) and hypercapnic (7% CO2) conditions. A bilateral injection of the immunotoxin anti-dopamine beta-hydroxylase-saporin (anti-D beta H-SAP; 2.4 ng/100 nl) or saline was performed in adult male Wistar rats (270-300 g, N = 5-8/group). Histology revealed a 60-75% loss of C1 neurons in anti-D beta H-SAP-treated rats, but no significant changes or C1 cell loss was observed in sham-treated rats or those with off-target injection sites. Bilateral depletion of C1 neurons did not alter cardiorespiratory variables during rest and hypercapnia (7% CO2), but it did affect the response to hypoxia. Specifically, the increase in ventilation, the number of sighs, and the tachycardia were reduced, but unexpectedly, the mean arterial pressure increased during hypoxia (8% 02). The present study indicates that C1 neurons contribute to cardiorespiratory control during hypoxia rather than at rest or during hypercapnia. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 15/23376-1 - Retrotrapezoid nucleus, respiratory chemosensitivity and breathing automaticity |
| Grantee: | Thiago dos Santos Moreira |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/22406-1 - Respiratory anatomofunctional changes observed in an experimental model of Parkinson Disease |
| Grantee: | Ana Carolina Thomaz Takakura |
| Support type: | Regular Research Grants |
| FAPESP's process: | 14/07698-6 - Contribution of catecholaminergic neurons of the rostral ventrolateral medulla in the respiratory control |
| Grantee: | Milene Rodrigues Malheiros Lima |
| Support type: | Scholarships in Brazil - Doctorate |