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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation

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Author(s):
Corchuelo, Sheryll ; Martinez, Emanuel R. M. ; Butzge, Arno J. ; Doretto, Lucas B. ; Ricci, Juliana M. B. ; Valentin, Fernanda N. ; Nakaghi, Laura S. O. ; Somoza, Gustavo M. ; Nobrega, Rafael H.
Total Authors: 9
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 450, n. C, p. 1-13, JUL 15 2017.
Web of Science Citations: 12
Abstract

Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/00423-6 - Paracrine and molecular regulation of the spermatogonial stem cell niche in zebrafish (Danio rerio) and the use of common carp (Cyprinus carpio) as a model to delay spermatogonial differentiation during puberty
Grantee:Rafael Henrique Nóbrega
Support Opportunities: Regular Research Grants
FAPESP's process: 14/02481-9 - Role of GnRH and GnRH receptors in the olfactory-retinal system in zebrafish and its relation with sexual preference and reproduction
Grantee:Sheryll Yohana Corchuelo Chavarro
Support Opportunities: Scholarships in Brazil - Master