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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ROLE OF A5 NORADRENERGIC NEURONS IN THE CHEMOREFLEX CONTROL OF RESPIRATORY AND SYMPATHETIC ACTIVITIES IN UNANESTHETIZED CONDITIONS

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Author(s):
Taxini, Camila L. ; Moreira, Thiago S. ; Takakura, Ana C. ; Bicego, Kenia C. ; Gargaglioni, Luciane H. ; Zoccal, Daniel B.
Total Authors: 6
Document type: Journal article
Source: Neuroscience; v. 354, p. 146-157, JUN 23 2017.
Web of Science Citations: 4
Abstract

The A5 area at the ventrolateral pons contains noradrenergic neurons connected with other medullary areas involved in the cardiorespiratory control. Its contribution to the cardiorespiratory regulation was previously evidenced in anesthetized conditions. In the present study, we investigated the involvement of the A5 noradrenergic neurons to the basal and chemoreflex control of the sympathetic and respiratory activities in unanesthetized conditions. A5 noradrenergic neurons were lesioned using microinjections of anti- dopamine beta-hydroxylase saporin (anti-D beta H-SAP). After 7-8 days, we evaluated the arterial pressure levels, heart rate and minute ventilation in freely moving adult rats (280-350 g) as well as recorded from thoracic sympathetic (tSN) and phrenic nerves (PN) using the arterially perfused in situ preparation of juvenile rats (8090 g). Baseline cardiovascular, sympathetic and respiratory parameters were similar between control (n = 7-8) and A5-lesioned rats (n= 5-6) in both experimental preparations. In adult rats, lesions of A5 noradrenergic neurons did not modify the reflex cardiorespiratory adjustments to hypoxia (7% O-2) and hypercapnia (7% CO2). In the in situ preparations, the sympatho-excitation, but not the PN reflex response, elicited by either the stimulation of peripheral chemoreceptors (Delta tSN: 110 +/- 12% vs 58 +/- 8%, P < 0.01) or hypercapnia (Delta tSN: 9.5 +/- 1.4% vs 3.9 +/- 1.7%, P < 0.05) was attenuated in A5-lesioned rats compared to controls. Our data demonstrated that A5 noradrenergic neurons are part of the circuitry recruited for the processing of sympathetic response to hypoxia and hypercapnia in unanesthetized conditions. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/06077-5 - Changes in the neural networks involved with the generation and control of sympathetic and respiratory activities in different experimental models of hypoxia
Grantee:Benedito Honorio Machado
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/17251-6 - Neural mechanisms generating the respiratory pattern and the respiratory-sympathetic coupling in conditions of hypoxia
Grantee:Daniel Breseghello Zoccal
Support type: Research Grants - Young Investigators Grants