| Full text | |
| Author(s): |
da Silva Teixeira, S.
;
Filgueira, C.
;
Sieglaff, D. H.
;
Benod, C.
;
Villagomez, R.
;
Minze, L. J.
;
Zhang, A.
;
Webb, P.
;
Nunes, M. T.
Total Authors: 9
|
| Document type: | Journal article |
| Source: | ACTA PHYSIOLOGICA; v. 220, n. 2, p. 238-250, JUN 2017. |
| Web of Science Citations: | 18 |
| Abstract | |
AimThyroid hormones regulate metabolic response. While triiodothyronine (T3) is usually considered to be the active form of thyroid hormone, one form of diiodothyronine (3,5-T2) exerts T3-like effects on energy consumption and lipid metabolism. 3,5-T2 also improves glucose tolerance in rats and 3,5-T2 levels correlate with fasting glucose in humans. Presently, however, little is known about mechanisms of 3,5-T2 effects on glucose metabolism. Here, we set out to compare effects of T3, 3,5-T2 and another form of T2 (3,3-T2) in a mouse model of diet-induced obesity and determined effects of T3 and 3,5-T2 on markers of classical insulin sensitization to understand how diiodothyronines influence blood glucose. MethodsCell- and protein-based assays of thyroid hormone action. Assays of metabolic parameters in mice. Analysis of transcript and protein levels in different tissues by qRT-PCR and Western blot. ResultsT3 and 3,5-T2 both reduce body weight, adiposity and body temperature despite increased food intake. 3,3-T2 lacks these effects. T3 and 3,5-T2 reduce blood glucose levels, whereas 3,3-T2 worsens glucose tolerance. Neither T3 nor 3,5-T2 affects markers of insulin sensitization in skeletal muscle or white adipose tissue (WAT), but both reduce hepatic GLUT2 glucose transporter levels and glucose output. T3 and 3,5-T2 also induce expression of mitochondrial uncoupling proteins (UCPs) 3 and 1 in skeletal muscle and WAT respectively. Conclusions3,5-T2 influences glucose metabolism in a manner that is distinct from insulin sensitization and involves reductions in hepatic glucose output and changes in energy utilization. (AU) | |
| FAPESP's process: | 10/18151-7 - Characterization of actions of TH and its agonist GC-1 in the homeostasis glycemic: study in muscle cells and diabetic rats |
| Grantee: | Silvania da Silva Teixeira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/17430-5 - Characterization of actions of T3 in the homeostasis glycemic |
| Grantee: | Silvania da Silva Teixeira |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 13/05629-4 - Genomic vs nongenomic actions of thyroid hormones: changes of paradigms, physiological implications and therapeutical perspectives |
| Grantee: | Maria Tereza Nunes |
| Support Opportunities: | Research Projects - Thematic Grants |