| Full text | |
| Author(s): |
Abriata, Juliana Palma
;
Eloy, Josimar O.
;
Riul, Thalita Bachelli
;
Campos, Patricia Mazureki
;
Baruffi, Marcelo Dias
;
Marchetti, Juliana Maldonado
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 77, p. 1196-1203, AUG 1 2017. |
| Web of Science Citations: | 6 |
| Abstract | |
Despite affecting millions of people worldwide, Chagas disease is still neglected by the academia and industry and the therapeutic option available, benznidazole, presents limited efficacy and side effects. Within this context, ursolic acid may serve as an option for treatment, however has low bioavailability, which can be enhanced through the encapsulation in polymeric nanoparticles. Therefore, herein we developed ursolic acid-loaded nano particles with poly-c-caprolactone by the nanoprecipitation method and characterized them for particle size, zeta potential, polydispersity, encapsulation efficiency, morphology by scanning electron microscopy and thermal behavior by differential scanning calorimetry. Results indicated that an appropriate ratio of organic phase/aqueous phase and polymer/drug is necessary to produce smaller particles, with low polydispersity, negative zeta potential and high drug encapsulation efficiency. In vitro studies indicated the safety of the formulation against fibroblast culture and its efficacy in killing T. cruzi. Very importantly, the in vivo study revealed that the ursolic acid loaded nanopartide is as potent as the benznidazole group to control parasitemia, which could be attributed to improved bioavailability of the encapsulated drug. Finally, the toxicity evaluation showed that while benznidazole group caused liver toxicity, the nanoparticles were safe, indicating that this formulation is promising for future evaluation. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/01515-1 - Potential use of nanostructured systems containing ursolic acid to optimize the therapy of Chagas Disease |
| Grantee: | JULIANA PALMA ABRIATA |
| Support Opportunities: | Scholarships in Brazil - Master |