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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new reconstructed human epidermis for in vitro skin irritation testing

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Author(s):
Pedrosa, Tatiana do Nascimento ; Catarino, Carolina Motter ; Pennacchi, Paula Comune ; de Assis, Silvia Romano ; Gimenes, Fabricia ; Lopes Consolaro, Marcia Edilaine ; de Moraes Barros, Silvia Berlanga ; Maria-Engler, Silvya Stuchi
Total Authors: 8
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 42, p. 31-37, AUG 2017.
Web of Science Citations: 6
Abstract

Different models of reconstructed human epidermis (RHE) are currently validated to assess skin irritation in vitro and ultimately to the animal replacement of the Draize test. The development of a new RHE model is a challenge for many laboratories, representing a potential gain of autonomy and improvement of technological knowledge. The Organization for Economic Co-operation and Development (OECD) encourages the development of new models and, for this purpose, offers a thorough guideline on quality control parameters (OECD TG 439 performance standards). This work aimed to develop an RHE model (i.e. USP-RHE) for in vitro skin irritation assays, following the OECD TG 439. The developed model presents a well-differentiated epidermis similar to the Validated Reference Methods (VRM) and to native human epidermis. Quality parameters, i.e. optical density of negative control, tissue integrity and barrier function, were similar to VRM and in accordance with OECD TG 439. Moreover, the USP-RHE model was shown to have 85,7% of specificity (6/7), 100% of sensitivity (6/6) and 92,3% of accuracy (12/13) when compared to in vivo UN GHS classification. The within-laboratory reproducibility was 92.3% (12/13). Thus, we demonstrated that USP-RHE model attends to all OECD TG 439 performance standards and is ready to be used by private and public laboratories and companies for future validation studies. (AU)

FAPESP's process: 11/07441-7 - Development of an artificial skin platform for risk assessment
Grantee:Silvya Stuchi Maria-Engler
Support type: Regular Research Grants
FAPESP's process: 11/14327-6 - Development of artificial skin containing glycated dermal equivalent in the assessment of efficacy and toxicity of compound anti-glycation
Grantee:Silvya Stuchi Maria-Engler
Support type: Regular Research Grants
FAPESP's process: 13/12682-9 - Immunosuppressive effects of UV radiation in immunocompetent epidermal equivalent
Grantee:Tatiana Do Nascimento Pedrosa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/24400-0 - In vitro models for pre clinical studies of melanoma Chemoresistant
Grantee:Silvya Stuchi Maria-Engler
Support type: Regular Research Grants
FAPESP's process: 11/22812-1 - Development of artificial skin containing glycated dermal equivalent mimicking “The pathophysiology OF skin aging ánd diabetes
Grantee:Paula Comune Pennacchi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/00735-0 - Development epidermis equivalent ón new biopolymeric membrane
Grantee:Carolina Motter Catarino
Support type: Scholarships in Brazil - Master