| Full text | |
| Author(s): |
Castro, Felipe Freitas
;
Ruy, Patricia C.
;
Zeviani, Karma Nogueira
;
Santos, Ramon Freitas
;
Toledo, Juliano Simoes
;
Cruz, Angela Kaysel
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Molecular and Biochemical Parasitology; v. 214, p. 69-74, JUN 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
Non-coding RNAs (ncRNAs) are regulatory elements present in a wide range of organisms, including trypanosomatids. ncRNAs transcribed from the untranslated regions (UTRs) of coding genes have been described in the transcriptomes of several eukaryotes, including Trypanosoma brucei. To uncover novel putative ncRNAs in two Leishmania species, we examined a L. major cDNA library and a L. donovani non-polysomal RNA library. Using a combination of computational analysis and experimental approaches, we classified 26 putative ncRNA in L. major, of these, 5 arising from intergenic regions and 21 from untranslated regions. In L. donovani, we classified 37 putative ncRNAs, of these, 7 arising from intergenic regions, and 30 from UTRs. Our results suggest, for the first time, that UTR-transcripts may be a common feature in the eukaryote Leishmania similarly to those previously shown in T. brucei and other eukaryotes. (AU) | |
| FAPESP's process: | 06/01252-0 - Estudo de rnas regulatorios em leishmania. |
| Grantee: | Karina da Costa Nogueira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/50219-9 - Studying the control of gene expression in Leishmania: post-translational modification, non coding RNAs, cis-elements and gene amplification |
| Grantee: | Angela Kaysel Cruz |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/16749-8 - Investigation of differentially expressed non-coding RNAs in Leishmania donovani |
| Grantee: | Ramon de Freitas Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/14184-0 - Antiinfective compounds: bringing together medicinal chemistry and organic synthesis in search of lead antiparasitic compounds |
| Grantee: | Flavio da Silva Emery |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/17025-3 - Investigation of noncoding RNAs involved in gene expression regulation in Leishmania |
| Grantee: | Felipe Freitas de Castro |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |