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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of methylene blue as photosensitizer in promastigotes of Leishmania major and Leishmania braziliensis

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Pinto, Juliana Guerra ; de Souza Martins, Jaciara Fagundes ; Correia Pereira, Andre Henrique ; Mittmann, Josane ; Raniero, Leandro Jose ; Ferreira-Strixino, Juliana
Total Authors: 6
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 18, p. 325-330, JUN 2017.
Web of Science Citations: 3

The cutaneous leishmaniasis is caused by the protozoan of the genus Leishmania. It is considered by WHO as a public health issue and a neglected disease, which affects rural workers and it is also a risk to travelers in endemic areas. The conventional treatment is toxic and leads to severe side effects. The photodynamic therapy has been studied as an alternative treatment to cutaneous leishmaniasis. This study aimed to evaluate the methylene blue internalization and the impact of the PDT in the viability and morphology of Leishmania major and Leishmania braziliensis promastigote in culture medium. The fluorescence microscopy was used to determine the MB localization. To evaluate the mitochondrial activity (MTT), viability (Trypan blue test) and the morphological alterations both species were incubated with the MB in concentrations starting in 500 mu g/ml, in serial dilution, until 7,8 mu g/ml. The fluorescence microscopy demonstrated that the MB is internalized by both species after one hour of incubation. The MB presented low toxicity at the dark and the PDT was capable of decreasing the viability in more than 70% in the higher concentrations tested. The PDT also triggered significant morphological alterations in the Leishmania promastigotes. The results presented in this study are an indicative that the MB is a photosensitizer with promising potential to clinical application, besides its low cost. (AU)

FAPESP's process: 13/12284-3 - Evaluation of photodynamic therapy action in Leishmania sp.: in vitro study
Grantee:Juliana Ferreira Strixino
Support Opportunities: Regular Research Grants