International high-risk clones of Klebsiella pneumoniae KPC-2/CC258 and Escherichia coli CTX-M-15/CC10 in urban lake waters

Author(s)

Nascimento, Tatiane ; Cantamessa, Rodrigo ; Melo, Luana ; Fernandes, Miriam R. ; Fraga, Edmir ; Dropa, Milena ; Sato, Maria I. Z. ; Cerdeira, Louise ; Lincopan, Nilton

• Document type: Journal article
• Source: Science of The Total Environment; v. 598, p. 910-915, NOV 15 2017.
• Web of Science Citations: 15

Abstract

The emergence of high-risk clones of multidrug-resistant (MDR) bacteria in aquatic environments has generated an important public health problem, creating an urgent need to strengthen surveillance. This study reports the occurrence of clinically significant MDR Enterobacteriaceae and non-fermentative bacteria carrying carbapenemases (KPC-2), extended-spectrum beta-lactamases (CTX-M) and plasmid-mediated quinolone resistance (PMQR) genes in urban lakes and reservoirs, in Southeastern Brazil. In this regard, the detection of hospital-associated lineages of KPC-2-producing Klebsiella pneumoniae belonging to the international clonal complex CC258 (STH) and CTX-M-15-producing Escherichia coli belonging to the international CC10 (ST617), in an urban lake, is reported for the first time. Whole genome sequencing (WGS) analysis of KPC-2-producing K. pneumoniae ST11 revealed that bla(KPC-2) gene was carried by an IncN plasmid on a Tn4401b element. This study support that aquatic environments with public access can act as reservoirs of clinically important MDR bacteria, constituting a potential risk to human and animal health. On the other hand, the detection of high-risk clones highlights the extra-hospital spread of clinically significant bacteria into urban aquatic environments. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/23588-0 - Prevalence and diversity of multidrug-resistant Gram-negative bacteria in public aquatic environment and drinking water sources in São Paulo State: clinical and environmental impact
• Grantee: Tatiane Nascimento
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/04725-0 - Monitoring of multidrug-resistant Gram-negative bacteria of medical importance (human & veterinary): clinical/environmental impact and development of therapeutic alternatives and technology innovation products
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants